FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754883

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754883
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences276387
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT5840.21129792645819084No Hit
TCCCTGGACCTTGCCGGACCCAGTGCATGGCATGATCTTCAAAAGTGAAT5620.20333807306421792No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT5350.19356916208070568No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC5270.19067466993744278No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT4530.16390061761226107No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT4050.14653366475268373No Hit
CTCCAGGCCCTTCCCTGGACCTTGCCGGACCCAGTGCATGGCATGATCTT3830.1385738113587108No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGTCAGGGGCCCTGTT3810.13785018832289508No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG3500.12663403126775138No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC3500.12663403126775138No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA3480.12591040823193564No Hit
GTCTGAGCCCTGACGACACGGCCTTGTATTTCTGTACAAGAGGGTCCGGTGGGGGGGCCCTACGACATATTGACT3430.12410135064239636No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT3360.12156867001704133No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC3300.11939780090959415No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA3210.11614149724842342No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGGAGCCTGAAGACT3150.11397062814097624No Hit
GTCCAGGGAAGGGCCTGGAGTGGGTCTCAGGTATTAATTGGAATAGTTAT3110.11252338206934479No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG3040.10999070144398976No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT3030.1096288899260819No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC3030.1096288899260819No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA2840.10275447108583256No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG2800.10130722501420111No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTTA150.002330611269.1713726
GGTATCA1850.048.6157041
TGTTAGA700.001843884836.9707970
GTATCAA3300.035.6401861
CCTTTCG400.00248296934.80613759
GGTAACA400.002541185434.64210547
TAGAGTG400.002563862134.5794265
TATCAAC3650.032.2109762
CCGTTCG450.00456503730.7372689
ATCAACG3900.030.1461663
GACCGAT1354.6341243E-528.7550670
TCAACGC4150.028.3301354
TGGACCG500.0076387227.6635445
CAACGCA4300.027.3418735
AGTCTCA1804.3655746E-1127.10929169
GGGAACG1207.866791E-426.95786770
TACGGGC906.7594483E-626.89511115
AACGCAG4400.026.7204696
TTCGGGG2206.755545E-826.46772670
AGAGTAC4600.026.31043211
Produced by FastQC (version 0.11.5)