FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754884

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754884
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences276387
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT6750.24422277458780622No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT5560.20116720395677076No Hit
TCCCTGGACCTTGCCGGACCCAGTGCATGGCATGATCTTCAAAAGTGAAT5110.184885685650917No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC4530.16390061761226107No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT4140.1497899684138545No Hit
GTCTGAGCCCTGACGACACGGCCTTGTATTTCTGTACAAGAGGGTCCGGTGGGGGGGCCCTACGACATATTGACT3700.1338702616259086No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA3630.13133758100055357No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT3500.12663403126775138No Hit
CTCCAGGCCCTTCCCTGGACCTTGCCGGACCCAGTGCATGGCATGATCTT3360.12156867001704133No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC3130.11324700510516052No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGGAGCCTGAAGACT3130.11324700510516052No Hit
GTCCAGGGAAGGGCCTGGAGTGGGTCTCAGGTATTAATTGGAATAGTTAT3010.10890526689026618No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC3000.10854345537235832No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA2990.10818164385445048No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT2880.10420171715746399No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT2820.10203084805001682No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGT150.002323103469.2276138
AAGCGTT150.0023415769.08955427
TAATTTG200.007226760551.9773646
GTATCAA3350.045.290141
GGTATCA1250.038.6201361
TATCAAC3900.038.0120282
TCAACGC4200.036.117744
CAACGCA4200.035.2968835
AACGCAG4500.034.476036
ATCAACG4300.034.4760253
AGAGTAC5000.031.04527511
CGCAGAG5050.030.054838
ACGCAGA5050.030.0439557
GCGTAGA2102.4881956E-829.33328270
CAGAGTA5450.028.48190310
GCAGAGT5750.026.995899
TAGGAGT2357.413655E-826.2127270
GTGGTAT809.569475E-525.86171
ACGGGGG1101.1453831E-625.09617616
AGTACGG5950.024.9289813
Produced by FastQC (version 0.11.5)