FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences956074
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTTTGATAGCTCACCGTACACTTTTGGCCAGGGGA31580.33030916016961026No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28940.3026962348102762No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA23020.24077634158025424No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA18470.19318588310109885No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18360.19203534454445995No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17750.18565508527582594No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17160.17948401483567172No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16710.17477726619487613No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT16710.17477726619487613No Hit
GTGTATTACTGTCAGCAGTTTGATAGCTCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA16360.17111646169647957No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16160.16902457341168153No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15680.16400404152816622No Hit
CATCTATGCTGCGTCCAGCAGGGCCACTGGCATCCCTGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA15620.16337647504272682No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG14830.15511351631777456No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCAGGGATGCCAGTGGCCCTGCT14820.15500892190353466No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14210.14862866263490063No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14000.1464321799358627No Hit
CTCCAGGCACGCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGACAGCA13530.1415162424665873No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13480.1409932703953878No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA13420.14036570390994837No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13350.13963354301026906No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA12520.13095220662835722No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGACAGCACCAACCTAGCCTGGTACC12080.12635005240180153No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11720.12258465348916506No Hit
GTCTCCAGGCACGCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGACAG11690.12227087024644537No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA11460.1198651987189276No Hit
GCATAGATGAGGAGCCTGGGAGCCTGGCCAGGCTTCAGCTGGTACCAGGCTAGGTTGGTGCTGTCAACACTCTGA11330.1185054713338089No Hit
GGCCAAAAGTGTACGGTGAGCTATCAAACTGCTGACAGTAATACACTGCA10840.1133803450360537No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10780.11275277855061429No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10620.11107926792277584No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTTTGATAGCTCA10570.11055629585157634No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10490.10971954053765713No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC10240.10710468018165957No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10000.10459441423990193No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9970.10428063099718224No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9740.10187495946966448No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18350.039.6989821
TATCAAC19750.036.7040252
GGTATCA8600.035.3938181
ATCAACG20650.034.9387473
TCAACGC21200.034.032324
CAACGCA21400.033.8740435
AACGCAG22100.032.8028266
TACGGGT1500.031.94410715
GAATACG450.004422711730.94456146
AGAGTAC22900.030.93937711
CGCAGAG23650.030.2413318
ACGCAGA24550.029.2582977
GCAGAGT24500.029.057039
CAGAGTA26300.026.67932310
AGTACGG22400.025.9763213
TACGGGA8500.025.77003515
CTCGTAT700.001150882525.27391660
GAGTACG23500.024.90605512
TAATATC700.001404828524.4240064
TGTTAGA6950.024.39830670
Produced by FastQC (version 0.11.5)