FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences617014
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT12520.20291273779849406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG2050.049.0151540
AGGGGGG3450.047.73383370
CTCGTAT2150.041.8560939
CTTATAC2500.039.7752461
GGTATCA12000.039.4323581
TTATACA2650.038.792582
CGTATGC2600.038.6877741
TATACAC2850.038.474983
GTATCAA26500.037.78261
GTGGTAT4500.037.3369141
TACACAT2800.036.71445
TCTCGTA2450.036.7038238
TGGTATC4550.035.3964462
ATACACA3300.035.305164
ACGAGAC2850.034.87642722
CGAGACA3050.034.8398223
TAGGTAC601.4053259E-534.2667773
TATCAAC30450.032.972632
ATCAACG32150.031.5488823
AATCTCG3100.031.18311936
Produced by FastQC (version 0.11.5)