FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005754894

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754894
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1134656
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC63490.5595528512606464No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC53010.46719005584071294No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT51340.4524719386316205No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT50650.4463908003835524No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG49140.4330828021885047No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG46190.40708373286705396No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC41950.3697155789948672No Hit
GACGTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCGGACCATTCAGA41420.3650445597608438No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG39550.3485637937841954No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC39270.346096085509617No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG38760.34160133115234925No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC37690.33217116024592475No Hit
GCCTTGGGCTGACGTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCGGACCATTCAGACTGTCATCCCATG37520.3306729087935022No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAACTTGGTCCCTC36470.3214190027638333No Hit
GCCCTGAGGGCCGCTGATTATTCCTATAAATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTGCTGGTACCAGGTCA35240.31057871284336397No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC33520.29541993344238254No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT33460.29489113881211576No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT33290.29339288735969316No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA31360.27638332675277794No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT31230.2752376050538666No Hit
CCTCAGGGCTCCCTGACCGGTTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGACTCCAGT29990.26430918269501946No Hit
GCATGGGATGACAGTCTGAATGGTCCGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTACGTCAGCCCAAGG29520.26016695809126295No Hit
ATAATAATCAGCCTCATCCCCAGACTGGAGTCCACTGATGGCCAGGGAGG29450.2595500310226183No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC28950.25514340910372835No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG28680.25276383326752777No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG28280.24923853573241583No Hit
GATTATTATTGTGCAGCATGGGATGACAGTCTGAATGGTCCGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTA28080.24747588696485984No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA27600.2432455299227255No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA27310.2406896892097693No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC27070.2385745106887021No Hit
GTTTGGGGGCCGTTCCTGGGAGTTGCTGGTACCAGGTCACAGAATTACCG26300.23178831293361157No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG26290.23170018049523378No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC26020.22932060465903323No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25250.22253440690394266No Hit
TCCCCAGACTGGAGTCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAACCGGTCAGGGAGC24600.21680579840938574No Hit
GTCATCCCATGCTGCACAATAATAATCAGCCTCATCCCCAGACTGGAGTC24420.2152194145185854No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA23680.20869761407862822No Hit
GGATGACAGTCTGAATGGTCCGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTACGTCAGCCCAAGGCTGCCCC23610.20808068700998364No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT23550.20755189237971686No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA23440.20658243555756106No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC23050.2031452704608269No Hit
ATCCCATGCTGCACAATAATAATCAGCCTCATCCCCAGACTGGAGTCCAC22840.2012944892548931No Hit
GTCTGGGGATGAGGCTGATTATTATTGTGCAGCATGGGATGACAGTCTGAATGGTCCGGTGTTCGGCGGAGGGAC22670.19979623780247052No Hit
ACGTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCGGACCATTCAGAC22550.19873864854193696No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22540.19865051610355916No Hit
TGGGATGACAGTCTGAATGGTCCGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTACGTCAGCCCAAGGCTGCC22330.19679973489762537No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT22020.1940676293079136No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG21720.1914236561565796No Hit
GTCCGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC21630.19063046421117943No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA21620.19054233177280164No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGACTCCAGTCTGGGGATG21030.18534251790851147No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA20850.1837561340177111No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG20800.18331547182582208No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG20350.17934951209882113No Hit
CTCTTGTTCTGGAAGCAGATCCAATATCGGCGGTAATTCTGTGACCTGGTACCAGCAACTCCCAGGAACGGCCCC19970.17600047944046476No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC19800.17450222798804219No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA19770.1742378306729088No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT19430.1712413277680636No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG19410.17106506289130802No Hit
CTGTCATCCCATGCTGCACAATAATAATCAGCCTCATCCCCAGACTGGAG19190.16912614924699645No Hit
GTGCCAGACTTGGAGCCAGAGAACCGGTCAGGGAGCCCTGAGGGCCGCTG19060.16798042754808507No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT18890.16648217609566246No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC18880.16639404365728466No Hit
GTTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGAC18860.16621777878052907No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC18300.16128236223137232No Hit
GTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGG18110.15960784590219415No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC17930.15802146201139375No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCGGAC17790.15678760787410456No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC17680.1558181510519488No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG17470.153967369846015No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT17370.153086045462237No Hit
ACACCGGACCATTCAGACTGTCATCCCATGCTGCACAATAATAATCAGCC17370.153086045462237No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17120.15088273450279205No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT17060.15035393987252524No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAGATCCAATATCGGCGGTAATTCT16880.14876755598172484No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC16620.14647611258390208No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC16620.14647611258390208No Hit
GTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGATCCAATATCGGCGGTAATTCTGTGAC16600.1462998477071465No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT16600.1462998477071465No Hit
GTGGACTCCAGTCTGGGGATGAGGCTGATTATTATTGTGCAGCATGGGAT16560.14594731795363527No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG16500.1454185233233685No Hit
GTGTTCGGCGGAGGGACCAAGTTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC16470.1451541260082351No Hit
TCAGTGGACTCCAGTCTGGGGATGAGGCTGATTATTATTGTGCAGCATGG16420.1447134638163461No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC16250.14321521236392354No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT16190.14268641773365673No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC16160.14242202041852334No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16110.14198135822663432No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAACCGGTC15990.14092376896610073No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA15820.13942551751367815No Hit
GCCCCCAAACTCCTCATTTATAGGAATAATCAGCGGCCCTCAGGGCTCCCTGACCGGTTCTCTGGCTCCAAGT15790.13916112019854476No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA15700.13836792825314456No Hit
GGCCATCAGTGGACTCCAGTCTGGGGATGAGGCTGATTATTATTGTGCAG15700.13836792825314456No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG15540.13695780923909978No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG15460.13625274973207738No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAAC15450.13616461729369958No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG15340.13519516047154378No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT15160.1336087765807434No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC15150.1335206441423656No Hit
GTCTGAATGGTCCGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCA14870.13105293586778724No Hit
CCCAGGAACGGCCCCCAAACTCCTCATTTATAGGAATAATCAGCGGCCCTCAGGGCTCCCTGACCGGTTCTCTGG14860.13096480342940944No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC14810.13052414123752046No Hit
ATTCAGACTGTCATCCCATGCTGCACAATAATAATCAGCCTCATCCCCAG14740.12990721416887585No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG14710.12964281685374246No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC14510.12788016808618646No Hit
GATTATTCCTATAAATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTGCTGG14340.12638191663376389No Hit
CTCCAGTCTGGGGATGAGGCTGATTATTATTGTGCAGCATGGGATGACAG14190.1250599300580969No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAACTTGGTCCCTCCGCCGAACAC14160.12479553274296351No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAACTTGGTC14010.12347354616729651No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC13900.12250408934514072No Hit
GGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13870.12223969203000733No Hit
GGAATAATCAGCGGCCCTCAGGGCTCCCTGACCGGTTCTCTGGCTCCAAG13850.12206342715325173No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13700.12074144057758476No Hit
GGAGTCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAG13540.11933132156353995No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT13510.11906692424840656No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT13500.11897879181002877No Hit
GTTCCTGGGAGTTGCTGGTACCAGGTCACAGAATTACCGCCGATATTGGA13260.11686361328896158No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGG13240.11668734841220599No Hit
GGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGT13190.116246686220317No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGA13160.11598228890518361No Hit
GGGCTCCCTGACCGGTTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCT13080.11527722939816121No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12920.11386711038411641No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAG12790.11272138868520504No Hit
GGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC12710.11201632917818263No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC12600.11104687235602684No Hit
CTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC12370.10901982627333746No Hit
GTGCAGCATGGGATGACAGTCTGAATGGTCCGGTGTTCGGCGGAGGGACC12270.10813850188955948No Hit
GTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAG12210.1076097072592927No Hit
GCTGGTACCAGGTCACAGAATTACCGCCGATATTGGATCTGCTTCCAGAA12010.10584705849173671No Hit
GATCCAATATCGGCGGTAATTCTGTGACCTGGTACCAGCAACTCCCAGGA11880.10470133679282533No Hit
GACGTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCGGACCATTCAGACTGTCATCCCATGCTGCACAATAAT11830.10426067460093631No Hit
GGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGG11830.10426067460093631No Hit
GGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGATCCAATATCGGCGGTAATTCTGTGACCTGGTACCAGCAA11640.10258615827175813No Hit
GTCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAACCGGTCAGGGAGCCCTGAGGGCCG11630.10249802583338033No Hit
ATGCTGCACAATAATAATCAGCCTCATCCCCAGACTGGAGTCCACTGATG11570.10196923120311355No Hit
CAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTG11440.10082350950420214No Hit
CCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTG11400.10047097975069096No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)