FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754895

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754895
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1325569
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31050.23423903244568933No Hit
GTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA28410.21432305674016217No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT24010.18112976389761679No Hit
GAGTTACAGTACCCCTCCATTCACTTTCGGCCCTGGGACCAAAGTGGATTTCAAACGAACTGTGGCTGCACCAT23100.1742647874233631No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC22540.17004018651613004No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT21560.16264713492847221No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21350.16106290958825983No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG21100.15917692704038794No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT20820.15706462658677142No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20160.15208563266038963No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20110.15170843615081522No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19830.1495961356971987No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG19700.14861542477230533No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCCAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA19650.14823822826273095No Hit
GTGCAGCCACAGTTCGTTTGAAATCCACTTTGGTCCCAGGGCCGAAAGTG19570.14763471384741195No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19550.1474838352435822No Hit
GTGTATTACTGTGCGAAATTACATTATCCGACTAGTGGATATTACTACGG19330.14582417060145492No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT19060.14378730944975326No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCCTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC18870.14235396271337064No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18580.14016622295783926No Hit
ACCTCATACTACGCAGACCCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCAAGAACACACTGTATCTG18460.13926095133486072No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG17920.13518722903145744No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16500.12447484815954507No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC16410.12379589444231119No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGATCATTAGCAA15950.12032568655422689No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG15790.1191186577235889No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15640.11798706819486575No Hit
GTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCCTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT15320.11557301053358973No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC15240.11496949611827072No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC15020.11330983147614344No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14790.1115747275321013No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14520.10953786638039967No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT14410.10870803405933602No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14060.10606765849231538No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC13960.10531326547316662No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGATCATTAGCAACTATTTAAATTGGTATCAGCAGAAA13820.10425711524635836No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT13740.10365360083103933No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA13680.10320096501955009No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13590.10252201130231621No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT13290.10025883224486995No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG200.00732761451.80646535
GTATCAA20600.041.661891
GTGGTAT3050.038.4225231
TATCAAC23100.037.2881662
TCAACGC25000.034.3164444
ATCAACG25200.034.1808173
CAACGCA25800.033.3859145
AACGCAG26600.032.3818246
TGTTAGA4700.031.57806870
AGAGTAC27850.030.92959211
AACGTAT450.004611970430.68156433
CGCAGAG29250.029.329198
GCAGAGT30300.028.4276089
CAGAGTA31050.027.74199910
CTCGTAT1254.0236046E-927.68140239
GTATAGA658.705923E-426.5132791
CGTATGC1057.4307536E-726.38723441
TCGTATG1057.455383E-726.3772340
ATTTGCA10800.025.29616738
GTAATAC11150.025.0390011
Produced by FastQC (version 0.11.5)