FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005754897

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754897
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1280226
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCAGAGCTGGAGCCTGAGAAGCGATGAGGGATCCCGTCCCCCAGGGT32710.2555017629699756No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAATCAATGCCAGTGGCCCATGTCTGACAGTAATAGT30940.2416760790672897No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAATCAATGCCAGTGGCCCATGTCTGA30780.24042629973145369No Hit
CTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACT26590.207697703374248No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC24760.19340335222062355No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG20500.16012797740398962No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG20360.1590344204851331No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTC19480.152160634138035No Hit
GCTCTGGGACTGAGCGCTACCTCACCATCTCCAGGCTCCAGTCTGAGGAT19300.1507546323852195No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT19230.15020785392579122No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCT19150.1495829642578732No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC18080.14122506494946985No Hit
CTCCTGTACAGCCTCTGGATTCATCTTTGGTTCCTATGCCATGAACTGGGCCCGCCAGGCTCCAGTAAAGGGG17420.13606972518914628No Hit
CACCTGCACTCTGGCCAGTGGGCACAGCACCTACGCCATCGCGTGGCTTC17250.1347418346448205No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16610.12974271730147646No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG16340.12763371467225318No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC16130.12599337929396842No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGC15790.12333759820531687No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG15090.1178698136110343No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14800.11560458856483152No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC14500.11326125231013899No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14020.10951191430263094No Hit
GGGTCTCTCTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCAC13640.10654368838002039No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13290.1038097960828791No Hit
CCACTGCACAGGGTCTCTCTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGT13230.1033411288319406No Hit
GGGCTGAACAGAGAGAAGTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT13150.1027162391640226No Hit
CCTGCACTCTGGCCAGTGGGCACAGCACCTACGCCATCGCGTGGCTTCAG13090.10224757191308409No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)