FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754898

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754898
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1280226
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCAGAGCTGGAGCCTGAGAAGCGATGAGGGATCCCGTCCCCCAGGGT29470.23019373141929628No Hit
CTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACT28780.22480405803350345No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAATCAATGCCAGTGGCCCATGTCTGA27600.21558693543171284No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAATCAATGCCAGTGGCCCATGTCTGACAGTAATAGT27170.21222815346665355No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25250.19723080143662133No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG20110.15708164027288932No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19430.15177007809558626No Hit
GCTCTGGGACTGAGCGCTACCTCACCATCTCCAGGCTCCAGTCTGAGGAT18170.14192806582587764No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT18010.1406782864900416No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCT17680.13810061660987982No Hit
CTCCTGTACAGCCTCTGGATTCATCTTTGGTTCCTATGCCATGAACTGGGCCCGCCAGGCTCCAGTAAAGGGG17640.13778817177592081No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC17200.13435127860237178No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16300.12732126983829417No Hit
CACCTGCACTCTGGCCAGTGGGCACAGCACCTACGCCATCGCGTGGCTTC16290.12724315862980443No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTC16250.12693071379584542No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGC15890.12411871029021437No Hit
GGGTCTCTCTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCAC15890.12411871029021437No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG15880.12404059908172464No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15060.11763547998556506No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14950.11677625669217778No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG13970.10912135826018218No Hit
CCACTGCACAGGGTCTCTCTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGT13830.10802780134132567No Hit
GGGCTGAACAGAGAGAAGTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT13520.10560635387814338No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT13460.10513768662720488No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC12830.10021668049235058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21000.042.942621
GTGGTAT2750.040.0519641
TATCAAC23750.037.8076632
TCAACGC25350.035.5543144
ATCAACG25350.035.418613
CAACGCA25650.035.0043565
AACGCAG26100.034.6657946
GGTATCA8800.034.4196551
AGAGTAC28500.031.03928811
CAGAGTA29100.030.75415810
GCAGAGT30350.029.8254229
CGCAGAG30350.029.8207638
TGGTATC3600.029.6252562
ACGCGAT7150.029.25445270
CGAGTAA500.00780883627.54649221
TATAAGA3000.026.3760362
GTATAAG3450.024.941781
ACGCAGA36150.024.937157
AGTACGG27600.024.31918313
GAGTACG27750.024.1877312
Produced by FastQC (version 0.11.5)