FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1015334
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT15740.15502287917079505No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16700.040.06231
CTTATAC3600.039.0750161
GTGGTAT6450.038.8312071
GTATCAA37800.037.9404341
TGGTATC6650.037.6355322
TACACAT4400.035.8428085
TATACAC4350.033.890353
TATCAAC44650.032.4031942
TTATACA4300.031.8924882
TCGTATG4250.031.79925740
CGTATGC4600.030.9125641
AACGCAG47600.030.3950146
ATCAACG48250.030.1987153
CTCGTAT3900.030.18329639
TCAACGC48450.029.932534
AGGGGGG6350.029.75965570
CAACGCA49000.029.5965545
AGAGTAC49900.028.10085911
CAGAGTA49850.028.0602710
ACGCAGA51650.027.945297
Produced by FastQC (version 0.11.5)