FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1052618
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAACCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT17820.1692921838691719No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT15700.14915192405982036No Hit
GGTTAGGAGACTGTCCTGGCTTCTGCTGGTACCAAGCTAAGTAGTTCTTACTACTGGAGCCGTATAAAACACT14390.1367067635172494No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12410.11789652086511915No Hit
GTTTTATACGGCTCCAGTAGTAAGAACTACTTAGCTTGGTACCAGCAGAA12400.11780151963960335No Hit
GTATGATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTC12210.11599649635480298No Hit
GAGTAGTATGATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAAT12000.11400147061897098No Hit
ATATCATACTACTCCGTTCACTTTCGGCCCTGGGACCAGAGTGGATATCAAACGAACTGTGGCTGCACCAT11200.10640137257770625No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11100.10545136032254818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4350.041.201081
TGGTATC4500.040.5801282
GTATCAA23750.036.8607861
GGTATCA11050.033.686511
TATCAAC26250.033.207892
TCAACGC27500.031.698444
ATCAACG27650.031.6510893
CAACGCA27700.031.3451845
AACGCAG28600.030.4792696
ACGCAGA31200.028.0497637
AGAGTAC30950.027.38704111
CGCAGAG32050.027.3058538
CAGAGTA31600.026.93274110
TCGTATG1306.044502E-926.64419740
GCAGAGT34000.025.6396629
CGTATGC1551.4515535E-924.58729241
AGTACGG31500.023.18974713
ACGATTG1052.15496E-523.16908647
GAGTACG31900.023.00697912
TATGCCG1755.837137E-921.79918143
Produced by FastQC (version 0.11.5)