FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754906

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754906
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367179
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16460.44828271769355No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGGAC200.00686112152.6676459
GGTATCA6050.038.0681651
GTAGTTT1205.0981726E-737.44584370
GTATCAA13850.037.2292251
TGGTATC2100.032.7202452
TATCAAC16200.031.811352
CAACGCA17850.029.2557495
GTGGTAT2350.029.2553061
AACGCAG17800.028.7588886
TCAACGC18150.028.5828934
ATCAACG18200.028.3155983
AGAGTAC18550.026.8662711
ACGCAGA19150.026.73157
CGCAGAG19200.026.4865578
CAGAGTA19050.026.1611210
GCAGAGT19800.025.690939
ACGGGTA951.0827289E-525.31513616
TCACGAC902.1833977E-422.9041713
AGTACGG21400.022.32463513
GAGTACG21700.021.8576112
Produced by FastQC (version 0.11.5)