FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754912

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754912
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences934020
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26220.28072204021327163No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36600.038.2433431
ATTAGAC451.0393882E-438.0918353
GTGGTAT7200.037.6418881
TGGTATC7750.034.5038342
GGTATCA16350.033.991791
TATCAAC44200.031.6455292
TCAACGC46950.029.7919564
CAACGCA46850.029.7091945
ATCAACG47550.029.488133
AACGCAG47350.029.4678786
AAAATCG900.006843459328.29809470
CAGAGTA48500.028.2955610
ACGCAGA49600.028.2769367
AGAGTAC48300.028.27066411
CGCAGAG50400.027.7009168
GCAGAGT50300.027.6907589
TAGTACC809.979564E-525.7119924
GTTAGCC1452.1536835E-823.6432113
AGTACGG49900.023.44519813
GAGTACG50700.023.27826112
Produced by FastQC (version 0.11.5)