FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences537330
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22250.41408445461820487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC2450.041.078692
GGTATCA8400.040.3708041
GTGTATA1500.040.2908631
GTATCAA20800.039.0640561
GTGGTAT2800.035.9739841
ATATCGG501.8817441E-434.43194637
TATCAAC24000.033.5475962
ATCAACG25400.031.4343643
CAACGCA25550.030.9872155
TCAACGC26350.030.3010584
GGTATAC450.005298392429.820093
AGAGTAC27700.028.73526811
CGCAGAG28100.028.546658
AACGCAG27950.028.3264166
GCAGAGT28550.028.1150269
CAGAGTA28550.027.87975310
CTCGCTA500.00871867126.92829123
ACGCAGA30200.026.667797
TGTATAA2300.026.254642
GGTAGAC908.57202E-626.0925773
Produced by FastQC (version 0.11.5)