FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005754919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1058492
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAATCAATGCCAGTGGCCCATGTCTGACAGTAATAGT41210.3893274583086126No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAATCAATGCCAGTGGCCCATGTCTGA34940.33009224443831414No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG33820.31951115360342824No Hit
GTCCCAGAGCTGGAGCCTGAGAAGCGATGAGGGATCCCGTCCCCCAGGGT33060.31233112767975574No Hit
CTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACT31280.29551475117431214No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC30790.2908855239340496No Hit
CTCCTGTACAGCCTCTGGATTCATCTTTGGTTCCTATGCCATGAACTGGGCCCGCCAGGCTCCAGTAAAGGGG29180.27567520585890115No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC27100.25602460859411313No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC26180.2473329982654569No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24590.23231162824093143No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG24250.22909951138034107No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC24050.22721003087411146No Hit
GCTCTGGGACTGAGCGCTACCTCACCATCTCCAGGCTCCAGTCTGAGGAT23640.22333659583634075No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT23300.22012447897575038No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21110.1994346674325361No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG21030.19867887523004427No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG21000.1983954531541098No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG20370.1924435895594865No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTC20280.1915933233316832No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCT20130.19017621295201098No Hit
CACCTGCACTCTGGCCAGTGGGCACAGCACCTACGCCATCGCGTGGCTTC19430.1835630311802073No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT18310.17298194034532144No Hit
GGGCTGAACAGAGAGAAGTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT17900.16910850530755075No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17790.16806929102912446No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGC16390.15484292748551712No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16380.15474845346020563No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC16340.15437055735895974No Hit
ACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAATCAATGCCAG16210.15314239502991048No Hit
CTCTGGGACTGAGCGCTACCTCACCATCTCCAGGCTCCAGTCTGAGGATG16200.153047921004599No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC16190.1529534469792875No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15830.1495523820680742No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG15830.1495523820680742No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGCCTGGAGATGGTGAGGTAGC15790.14917448596682828No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15420.14567894703030349No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15300.1445452587265657No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT15290.14445078470125425No Hit
GACTATTACTGTCAGACATGGGCCACTGGCATTGATTGGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTAGGT15130.14293920029627055No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14990.1416165639419098No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC14780.1396326094103687No Hit
CCTGCACTCTGGCCAGTGGGCACAGCACCTACGCCATCGCGTGGCTTCAG14740.13925471330912279No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT14630.1382154990306965No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG14470.13670391462571282No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14340.13547575229666356No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14100.133208375689188No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC14090.13311390166387654No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13620.12867362247423694No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC13600.12848467442361397No Hit
CCACTGCACAGGGTCTCTCTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGT13570.1282012523476795No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13540.12791783027174508No Hit
TCCTCAGACTGGAGCCTGGAGATGGTGAGGTAGCGCTCAGTCCCAGAGCTGGAGCCTGAGAAGCGATGAGGGATC13310.125744927689581No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13250.12517808353771215No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13000.12281623290492513No Hit
GTCTGAGGATGAGGCTGACTATTACTGTCAGACATGGGCCACTGGCATTG12960.12243833680367919No Hit
GCCACACCCTGGGGGACGGGATCCCTCATCGCTTCTCAGGCTCCAGCTCTGGGACTGAGCGCTACCTCACCATCT12770.12064333032276106No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12750.1204543822721381No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12720.12017096019620367No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12350.11667542125967886No Hit
GGGTCTCTCTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCAC12220.11544725893062961No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG12110.11440804465220333No Hit
GCACAGGGTCTCTCTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAG12090.11421909660158036No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA12060.11393567452564592No Hit
GGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGT11970.1130854082978426No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT11970.1130854082978426No Hit
CCCTGGGAGCCTCGGTCAAGCTCACCTGCACTCTGGCCAGTGGGCACAGC11860.1120461940194163No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC11780.11129040181692446No Hit
ATATTACGCAGACTCCGTGAAGGGCCGCTTCACCATCTCCAGAGACAATT11770.11119592779161298No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA11770.11119592779161298No Hit
CCCTGGGGGACGGGATCCCTCATCGCTTCTCAGGCTCCAGCTCTGGGACTGAGCGCTACCTCACCATCTCCAGG11770.11119592779161298No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11700.11053460961443261No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG11670.11025118753849815No Hit
ATCAATGCCAGTGGCCCATGTCTGACAGTAATAGTCAGCCTCATCCTCAG11630.10987329143725225No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC11590.10949539533600633No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGA11420.10788933690571115No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG11400.10770038885508817No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC11390.10760591482977669No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA11370.10741696677915374No Hit
CTCCAGGCTCCAGTCTGAGGATGAGGCTGACTATTACTGTCAGACATGGGCCACTGGCATTGATTGGGTGTTCGG11310.10685012262728484No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11130.10514959017167819No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC11010.10401590186794044No Hit
CTACCTCACCATCTCCAGGCTCCAGTCTGAGGATGAGGCTGACTATTACT10640.10052036293141564No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)