FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754920

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754920
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1058492
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAATCAATGCCAGTGGCCCATGTCTGACAGTAATAGT36440.34426334823503624No Hit
CTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACT36130.34133465345038033No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC31740.2998605563386403No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAATCAATGCCAGTGGCCCATGTCTGA30940.2923026343137218No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG29840.2819104915294589No Hit
GTCCCAGAGCTGGAGCCTGAGAAGCGATGAGGGATCCCGTCCCCCAGGGT29790.2814381214029015No Hit
CTCCTGTACAGCCTCTGGATTCATCTTTGGTTCCTATGCCATGAACTGGGCCCGCCAGGCTCCAGTAAAGGGG28820.27227414094768787No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT26420.24960037487293243No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG24790.23420110874716105No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC23390.22097474520355373No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23370.22078579715293076No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT22970.21700683614047153No Hit
GCTCTGGGACTGAGCGCTACCTCACCATCTCCAGGCTCCAGTCTGAGGAT22060.20840969983712676No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT21890.2068036414068316No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC21570.20378047259686421No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG20160.1904596350279454No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG19180.1812011805474203No Hit
CACCTGCACTCTGGCCAGTGGGCACAGCACCTACGCCATCGCGTGGCTTC18620.17591063512997737No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTC18420.17402115462374776No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCT18370.17354878449719033No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGC17820.1683527131050589No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17820.1683527131050589No Hit
CTCTGGGACTGAGCGCTACCTCACCATCTCCAGGCTCCAGTCTGAGGATG17800.16816376505443595No Hit
GGGCTGAACAGAGAGAAGTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT17770.1678803429785015No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG17300.1634400637888619No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16600.15682688201705824No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16440.15531529761207452No Hit
GGGTCTCTCTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCAC16430.15522082358676306No Hit
CCACTGCACAGGGTCTCTCTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGT16080.15191423270086124No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG15490.14634026520748386No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC15330.14482868080250016No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC15300.1445452587265657No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15090.14256130419502464No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14610.13802655098007355No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT14280.13490890814479467No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14270.13481443411948318No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC14170.13386969386636838No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT14010.13235810946138468No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG13890.13122442115764693No Hit
GACTATTACTGTCAGACATGGGCCACTGGCATTGATTGGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTAGGT13850.130846525056401No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13560.12810677832236805No Hit
ACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAATCAATGCCAG13520.12772888222112214No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13440.12697309001863027No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13290.12555597963895807No Hit
CCTGCACTCTGGCCAGTGGGCACAGCACCTACGCCATCGCGTGGCTTCAG13220.12489466146177769No Hit
GTCTGAGGATGAGGCTGACTATTACTGTCAGACATGGGCCACTGGCATTG13050.12328860303148252No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13040.12319412900617104No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT13030.12309965498085956No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA12990.12272175887961365No Hit
GCACAGGGTCTCTCTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAG12840.12130464849994142No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT12580.11884832384184292No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT12570.11875384981653145No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC12560.11865937579121996No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12550.11856490176590849No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGCCTGGAGATGGTGAGGTAGC12520.11828147968997403No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12400.11714779138623627No Hit
GCCACACCCTGGGGGACGGGATCCCTCATCGCTTCTCAGGCTCCAGCTCTGGGACTGAGCGCTACCTCACCATCT12350.11667542125967886No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC12310.11629752515843295No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12150.11478594075344925No Hit
GTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACTCTGGCCAGTGGG12090.11421909660158036No Hit
TCCTCAGACTGGAGCCTGGAGATGGTGAGGTAGCGCTCAGTCCCAGAGCTGGAGCCTGAGAAGCGATGAGGGATC12060.11393567452564592No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12000.11336883037377703No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT11990.11327435634846555No Hit
CCCTGGGGGACGGGATCCCTCATCGCTTCTCAGGCTCCAGCTCTGGGACTGAGCGCTACCTCACCATCTCCAGG11880.11223514207003926No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT11850.11195171999410483No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG11650.11006223948787519No Hit
ATATTACGCAGACTCCGTGAAGGGCCGCTTCACCATCTCCAGAGACAATT11510.10873960313351448No Hit
GGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGT11460.10826723300695706No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11440.1080782849563341No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11400.10770038885508817No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA11260.10637775250072745No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC11080.1046772200451208No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC11030.10420484991856338No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10750.10155957720984193No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10670.10080378500735007No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC10640.10052036293141564No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG10610.10023694085548118No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2050.040.321861
GGTATCA6200.037.7746471
TGGTATC2200.037.560222
GTATCAA13150.037.191681
TATCAAC14400.034.1911052
ATCAACG15600.031.338833
ACTTTCA11950.030.63124570
TCAACGC16200.030.1781334
GGGTTTA5000.029.6197661
CAACGCA16750.028.9816675
AACGCAG17000.028.7579866
GTTCTAA605.4759363E-428.7013241
TCGCTAT605.4759363E-428.7013241
CGACATT500.00718409328.02070462
TATATGC500.0078132627.5428585
GTGTCCA10050.027.3166770
GGGCAGT3100.026.6644571
TAGAGTG658.7620306E-426.483525
CGCAGAG18600.026.4780378
CAGAGTA18500.026.43749610
Produced by FastQC (version 0.11.5)