FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences527410
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22190.4207352913293263No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTTT350.005821906554.89748470
GTATCAA24650.038.721691
GGTATCA10250.035.3792841
AACGCAG28950.032.591036
TATCAAC29300.032.3183862
CCATACG800.003735272432.02353770
GTCTAAA652.4563562E-531.5794771
CAACGCA30150.031.4072555
ATCAACG30550.031.1079333
AGAGTAC29600.031.09049211
CAGAGTA29500.030.84797510
TCAACGC31000.030.7666384
ACGCAGA31150.030.4105287
ATATAAG450.004832352530.38683
GTGGTAT3950.030.3136351
CGCAGAG31850.029.6376068
GCAGAGT32000.029.3973739
GCTTAGA605.691517E-428.509251
TGGTATC4150.027.1833742
AGTACGG31200.025.87762813
Produced by FastQC (version 0.11.5)