FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428196
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT4370.10205606778204374No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC2300.049.3147662
GTGGTAT2550.044.495571
CTTATAC950.043.4310871
TTATACA1000.041.245082
TCGTATG1050.039.57216640
GGTATCA5450.039.114541
CTCGTAT1009.094947E-1238.0792539
GTATCAA11750.037.162841
CGTATGC1151.8189894E-1236.15237441
GTATGCC1250.036.06158442
TATAGAG400.002642661834.37095
CTCGATA750.003077634633.31273370
TATACAC1450.033.1856963
TACACAT1400.031.9158365
TATCAAC13900.031.4036272
AGGGGGG2051.4315447E-930.46896270
ATCAACG14600.030.1333893
ATTCTAA802.830302E-630.0850751
CAACGCA14750.029.826955
TCAACGC14800.029.7261834
Produced by FastQC (version 0.11.5)