FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754928

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754928
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1288109
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAACATTGGATGACAGCCTGCCTAGTTGGGTGTTCGG70950.5508074239058962No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC62810.48761401403142124No Hit
GTCCTGGGCCCAGTCTGTTCTGACGCAGCCACCCTCAGCCTCTGGGACCC58680.45555151000419997No Hit
GCCTTGGGCTGACCCAGGACGATCAACTTGGTCCCTCCGCCGAACACCCA50800.3943765628529884No Hit
GTAATAATCAGCCTCATCTTCGGACCGGAGACCGCTGATGGCCAGGGAGG47810.3711642415354601No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTTCTGACGCAGCCACCCTCAGCCTCT47120.3658075520006459No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT39170.3040891725777865No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC34440.26736867765072675No Hit
GTTCTGACGCAGCCACCCTCAGCCTCTGGGACCCCCGGACAGAGGGTCAC33830.26263305356922434No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG33170.25750926357940207No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTTCTGACGCAGCCAC32980.25603423312778656No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG32960.2558789667644586No Hit
CCCCAGGGACGGCCCCCAAACTCCTCATCTATGGGAGTGAACAGCGGCCC32930.25564606721946664No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31960.2481156485980612No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG30450.23639303816680113No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT29450.22862972000040369No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA28660.22249669864894975No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGATCAACTTGGTCCCTC27530.2137241491209207No Hit
GTGGTAGAGAAGACAGGATTCAGGGCAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA26860.2085227259494344No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC26250.203787101867932No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25990.20176863914466867No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24500.19020129507673653No Hit
CCCTCAGCCTCTGGGACCCCCGGACAGAGGGTCACCATCTCTTGTTCTGG24410.18950259644176073No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG23600.18321430872697886No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGCTTGGAGGGTGTGGTGGTCTCCA22640.17576152328723735No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT22350.1735101610189821No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC22250.17273382920234234No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA22230.1725785628390144No Hit
ACATTGGATGACAGCCTGCCTAGTTGGGTGTTCGGCGGAGGGACCAAGTTGATCGTCCTGGGTCAGCCCAAGG22050.17118116556906288No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGGCAATCTCCAGCATGGCCGG20850.161865183769386No Hit
CCACCACACCCTCCAAGCAAAGCAACAACAAGTACGCGGCCAGCAGCTAC20320.15775062514119537No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19390.15053073924644578No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19260.1495215078848141No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGCGGTCTCCGGT19250.14944387470315013No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTT19100.14827937697819052No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC19090.14820174379652654No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT19040.14781357788820668No Hit
GGAGTGAACAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG18720.1453293160749595No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT18700.14517404971163156No Hit
CTCTTGTTCTGGAAGCGACTCCAACATCGGAGCTAATTATGTCTACTGGTACCAGCAATCCCCAGGGACGGCCCC18360.14253452153505644No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18080.14036079244846514No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGCTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17860.1386528624518577No Hit
GCCTAGTTGGGTGTTCGGCGGAGGGACCAAGTTGATCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACT17790.1381094301802099No Hit
GCTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16830.1306566447404684No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC16720.1298026797421647No Hit
GCCCCCAAACTCCTCATCTATGGGAGTGAACAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT16590.12879344838053303No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC16580.12871581519886904No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT16570.12863818201720506No Hit
GACCCAGGACGATCAACTTGGTCCCTCCGCCGAACACCCAACTAGGCAGG16500.12809474974555726No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC16450.12770658383723738No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGCGGTCTCCGGTCCGAAGATG16450.12770658383723738No Hit
GTGCAACATTGGATGACAGCCTGCCTAGTTGGGTGTTCGGCGGAGGGACC16220.12592102065896596No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG15570.12087486385080766No Hit
ATCCAATGTTGCACAGTAATAATCAGCCTCATCTTCGGACCGGAGACCGC15240.11831296885589651No Hit
CCCCCGGACAGAGGGTCACCATCTCTTGTTCTGGAAGCGACTCCAACATC15200.11800243612924062No Hit
GGGTCACCATCTCTTGTTCTGGAAGCGACTCCAACATCGGAGCTAATTAT14910.11575107386098536No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14790.11481947568101768No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA14780.1147418424993537No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC14750.11450894295436179No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC14700.11412077704604191No Hit
ATCTATGGGAGTGAACAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC14510.1126457465944264No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT14410.11186941477778667No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG14140.10977331887285935No Hit
CTATGGGAGTGAACAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC14050.1090746202378836No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC14040.10899698705621962No Hit
GGCCATCAGCGGTCTCCGGTCCGAAGATGAGGCTGATTATTACTGTGCAA13850.1075219566046041No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTTCTGACGCAGCCACCCTCAGCCTCTGGGACCCCCGGACAGAGGGTCAC13820.1072890570596122No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA13560.10527059433634886No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGATCAACTTGGTCCCTCCGCCGAACACCCAA13510.10488242842802899No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGCTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG13240.1027863325231017No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11200.042.4234431
GTATCAA27750.042.004951
TATCAAC30750.037.7854732
TCAACGC32450.035.5953334
GCCAGAG18650.035.09316370
ATCAACG33000.035.002083
CAACGCA33300.034.7893685
AACGCAG34000.033.9739276
ACGCAGA38100.030.5941587
CGCAGAG38400.030.5367348
AGAGTAC38200.030.25736611
CAGAGTA38550.029.98265510
GCAGAGT39450.029.7308989
CCGATAA500.00720538728.00416850
GTGGTAT3350.027.5504071
AATGTTG27100.027.4334770
AGTACGG26700.027.15203513
TACGGGT2050.026.68867715
GAGTACG27350.026.63177112
TGGTATC3500.026.3625132
Produced by FastQC (version 0.11.5)