FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences558046
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGGCTGTGTCTGTAGATGTGTCCTCGGT18130.3248836117452683No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT14980.2684366521756271No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA13240.23725642688953957No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12780.2290133788254015No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT12300.22041193736717044No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT12030.2155736265469155No Hit
GTGTATTACTGTGCAACAGCGGGATTTGTTGGGCAGTGGCTGGTACCTCT11900.21324406948531124No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGACCTCCAGCAACTTCGGCACCCAGAC11300.20249226766252243No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT10690.19156126914268717No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10120.1813470574110378No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG9730.17435838622622507No Hit
GGGTGCGACAGGCTCCTGGAAAAGGGCTTGAGTGGATGGGAGGTTTTGATCCTGAAAATGGTGAAACAATCTACG9280.16629453485913348No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8670.1553635363392982No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC8590.15392996276292634No Hit
GTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA8540.15303397927769394No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8430.15106281561018267No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT8380.15016683212495027No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC8230.14747888166925308No Hit
ATCCATGCACTGGGTGCGACAGGCTCCTGGAAAAGGGCTTGAGTGGATGG8120.1455077180017418No Hit
CCTCCATGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGGCTCACGTCCACCACCACGCACGTGA7990.14317816094013755No Hit
AGCTGGTACAGTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGTATCCGGAGACA7940.14228217745490515No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC7790.13959422699920795No Hit
CTATTATAGCGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC7760.1390566369080685No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGGGCTTGCTGGCCGTGGCACTCATTTA7760.1390566369080685No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACAAGACAGTTGAGCGCAAATGTTGTGT7720.1383398501198826No Hit
GGTCTAGAGGTACCAGCCACTGCCCAACAAATCCCGCTGTTGCACAGTAA7600.13618948975532483No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTT7530.1349351128759995No Hit
GAGTATTAGTAGCTGGTTGGCCTGGTTTCAGCAGAAACCAGGGAAAGCCCCTCAGCTCCTGATCTATAAGGCGT7340.13153037563211636No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTT7280.13045519544983747No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG7250.12991760535869804No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT7170.12848403178232617No Hit
GCATGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAACAGCACGTTCCGTGTGGTCAGCGTC7160.1283048350852797No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7160.1283048350852797No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7140.12794644169118677No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA7000.12543768793253604No Hit
GTATCCGGAGACACCCTCACTGAATTATCCATGCACTGGGTGCGACAGGC6920.12400411435616418No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGAA6580.11791142665658386No Hit
CCGCTGGTCAGAGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA6570.11773222995953739No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT6520.11683624647430499No Hit
GCCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGTGCAACAGCGGGATTTGTTGGG6330.11343150923042186No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG6280.11253552574518946No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6280.11253552574518946No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCG6170.11056436207767818No Hit
GAATTATCCATGCACTGGGTGCGACAGGCTCCTGGAAAAGGGCTTGAGTG6150.11020596868358522No Hit
GTCATGGTGACTCTGCCCTGGAACTTCTGTGCGTAGATTGTTTCACCATTTTCAGGATCAAAACCTCCCATCCA6110.10948918189539932No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAACCAAAGGGCAG6080.10895159180425987No Hit
GTCTAGAGGTACCAGCCACTGCCCAACAAATCCCGCTGTTGCACAGTAAT6050.10841400171312042No Hit
GTCTTCCCCCTGGCGCCCTGCTCCAGGAGCACCTCCGAGAGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA5980.10715962483379507No Hit
CTGTAGATGTGTCCTCGGTCATGGTGACTCTGCCCTGGAACTTCTGTGCGTAGATTGTTTCACCATTTTCAGGAT5910.10590524795446972No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT5900.10572605125742321No Hit
ACCATGGACTGCACCTGGAGGATCCTCTTCTTGGTGGCAGCAGCTACAGGCACCCACGCCCAGGTCCAGCTGGTA5850.10483006777219081No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG5820.10429247768105139No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG5810.1041132809840049No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT5790.10375488758991194No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCCAGTCAGA5740.10285890410467954No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCACTGAATCTTGATGGGACCCCA5700.10214211731649361No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACGT205.8148167E-498.12417670
TTATAGT300.002908039465.41611570
GTAGTTT503.3366567E-452.3328970
ACTAACC200.00742755551.6253282
AGTTATA306.1589165E-446.22471243
GTATCAA8650.041.405811
TATCAAC9100.038.9553762
ATCAACG9400.037.7121163
TCAACGC9450.037.509224
CCTTTGT6150.037.22868370
AAGTTAC400.001838441137.01050668
TGGTATC1500.036.711342
CAACGCA9750.036.355095
AACGCAG10050.034.9274376
GTGGTAT1600.034.4384881
ACGCAGA10700.032.81457
CGCAGAG11050.031.7893738
AGAGTAC10700.031.5446211
ACCGAAA450.00446812230.87779446
AACCGAA450.00447603830.86662945
Produced by FastQC (version 0.11.5)