FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences939464
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT55680.5926783783093338No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42650.45398227074161435No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC41890.4458925514974496No Hit
GACTAACAGTATCCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAGTCAAGCGAACTGTGGCTGCACCAT41150.43801571960181546No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC36450.3879871926971124No Hit
CTGTTAGTCTGCTGACAGTAGTAAGTTGCAAAGTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC36210.38543254451474457No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34780.370211099094803No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33750.35924740064547445No Hit
ACAGTATCCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAGTCAAGCGA33340.3548832100005961No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31200.33210426370781637No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31000.3299753902225099No Hit
GGATACTGTTAGTCTGCTGACAGTAGTAAGTTGCAAAGTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT29600.31507327582536426No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27880.29676496385172824No Hit
GTGCAGCCACAGTTCGCTTGACTTCCACCTTGGTCCCCTGGCCGAACGTC27830.29623274548040157No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27620.29399742832082976No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27170.2892074629788901No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25310.2694089395655395No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG24750.2634480938066812No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24640.2622772133897627No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22990.2447140071359839No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22910.24386245774186133No Hit
GTAGGAGACAGAGTCACCATCAATTGCCGGACAAGTCAGAGTGTTAGGAGCTATTTAAACTGGTATCAGCAGAAA22370.2381144993315337No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG22170.2359856258462272No Hit
GTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTCGATGGGAC21230.22597992046528662No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC21040.22395749065424542No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCAATTGCCGGACAAGTCAGAGTGTTAGGAG20790.22129639879761223No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT20240.21544199671301936No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT19890.21171646811373293No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18750.19958188924748577No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18500.19692079739085264No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17780.18925685284374918No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17670.18808597242683062No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17640.18776664140403462No Hit
CCTGAAGACTTTGCAACTTACTACTGTCAGCAGACTAACAGTATCCCGTG17510.1863828736385854No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGACTTCCACCTTGGTCCCCTGGCCGAACGTCCACGGGATA16940.18031558420546184No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16930.18020914053119652No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16870.17957047848560456No Hit
CACCATCAGCAGTCTGCAACCTGAAGACTTTGCAACTTACTACTGTCAGCAGACTAACAGTATCCCGTGGACGTT16680.17754804867456336No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC16540.1760578372348488No Hit
ACTTACTACTGTCAGCAGACTAACAGTATCCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAGTCAAGCGAA16300.173503189052481No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16190.1723323086355624No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15950.1697776604531946No Hit
GTAGTAAGTTGCAAAGTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA15800.1681810053392147No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15630.16637146287670415No Hit
GTCTGCAACCTGAAGACTTTGCAACTTACTACTGTCAGCAGACTAACAGTATCCCGTGGACGTTCGGCCAGGGGA15420.1641361457171323No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTCG14610.15551420810164093No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14600.1554077644273756No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTCGATGGGACCCCA14580.15519487707884497No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14400.1532788909420691No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCAATTGCCGGACAAGTCAGA14370.15295955991927312No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14200.15115001745676257No Hit
GGCTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGTTTAAATAGCTCCTA14120.15029846806264No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14010.1491275876457214No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGACTTTGCAACTTACTACTGTCAGCAGACTAACAGTATC13910.14806315090306812No Hit
GTGTTAGGAGCTATTTAAACTGGTATCAGCAGAAACCAGGGAAAGCCCCTAGCCTCCTAATCTATCGTGCATCCA13630.145082728023639No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13610.14486984067510836No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT13470.1433796292353938No Hit
GTCCCAGATCCACTGCCACTGAACCTCGATGGGACCCCACTTTGTAAAGT13390.1425280798412712No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT13340.14199586146994456No Hit
CTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCT13310.1416765304471486No Hit
CCTAATCTATCGTGCATCCACTTTACAAAGTGGGGTCCCATCGAGGTTCAGTGGCAGTGGATCTGGGACAGATTT13280.1413571994243526No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC13260.14114431207582198No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13200.14050565003023No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13090.1393347696133114No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC12990.13827033287065815No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12920.13752522715080087No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12900.13731233980227023No Hit
GCCACAGTTCGCTTGACTTCCACCTTGGTCCCCTGGCCGAACGTCCACGG12610.13422547324857578No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12510.13316103650592254No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12510.13316103650592254No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT12370.13167082506620797No Hit
CCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAGTCAAGCGAACTGTGG12280.13071283199782002No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT12190.1297548389294321No Hit
ATAGATTAGGAGGCTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGTTTAAATAGCTCCTAACACTCTGACTTGT12030.1280517401411869No Hit
GGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCC11970.12741307809559493No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11960.1273066344213296No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11900.12666797237573765No Hit
GATTAGGAGGCTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGTTTAAAT11880.126455085027207No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11830.12592286665588037No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11690.12443265521616582No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11650.12400688051910451No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11620.12368754949630854No Hit
CAGTAGTAAGTTGCAAAGTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA11600.12347466214777789No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC11540.12283600010218593No Hit
ATTTAAACTGGTATCAGCAGAAACCAGGGAAAGCCCCTAGCCTCCTAATC11500.12241022540512463No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11280.12006846457128745No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC10890.11591716127493976No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCA10860.11559783025214378No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGACTTTGCAACTTA10840.11538494290361312No Hit
CCATCAGCAGTCTGCAACCTGAAGACTTTGCAACTTACTACTGTCAGCAG10660.11346895676683726No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG10570.11251096369844933No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10450.11123363960726543No Hit
AATCTATCGTGCATCCACTTTACAAAGTGGGGTCCCATCGAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA10440.11112719593300009No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10400.11070142123593878No Hit
CCCCTAGCCTCCTAATCTATCGTGCATCCACTTTACAAAGTGGGGTCCCA10140.10793388570504032No Hit
GTGGGGTCCCATCGAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT10080.10729522365944837No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG10040.10686944896238705No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGT9950.10591145589399914No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG9750.1037825824086926No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9740.1036761387344273No Hit
GTCCACGGGATACTGTTAGTCTGCTGACAGTAGTAAGTTGCAAAGTCTTC9650.10271814566603936No Hit
GGACCAAGGTGGAAGTCAAGCGAACTGTGGCTGCACCATCTGTCTTCATC9640.10261170199177404No Hit
CAGTATCCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAGTCAAGCGAACTGTGGCTGCACCATCTGTCTTCAT9550.1016537089233861No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9480.10090860320352882No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC9430.1003763848322022No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATGT150.002399528268.6778328
GTATCAA12550.043.9725571
CGTATGC1000.041.55027441
TCGTATG1000.041.51900540
CTCGTAT1000.041.4721939
ACCGTAC1000.041.1913227
GTACTAG1100.040.60618230
CTAGATC1250.038.53153233
TACTAGA1200.037.2382431
TATCAAC14900.036.5616232
TTCGGGG3200.036.0665170
TCAACGC15450.035.2600754
CAACGCA15650.034.8094675
ATCAACG15650.034.8094673
TTACATA400.002588942934.51938638
ACTAGAT1300.034.38293532
GACCGTA1300.034.3187826
ACGAGAC1201.8189894E-1234.28222322
AACGCAG16050.033.9419446
TATACAC1850.033.3361473
Produced by FastQC (version 0.11.5)