FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754932

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754932
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences939464
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT52120.5547844302708779No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC43590.463987976122555No Hit
GACTAACAGTATCCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAGTCAAGCGAACTGTGGCTGCACCAT38930.41438522391491317No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33200.3533929985608815No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33180.3531801112123509No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32950.35073190670424836No Hit
CTGTTAGTCTGCTGACAGTAGTAAGTTGCAAAGTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC32150.34221641276302234No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31930.33987465192918515No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT31170.3317849326850204No Hit
ACAGTATCCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAGTCAAGCGA30360.323162995069529No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC30230.32177922730407976No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26210.2789888702494188No Hit
GTGCAGCCACAGTTCGCTTGACTTCCACCTTGGTCCCCTGGCCGAACGTC25730.2738795738846832No Hit
GGATACTGTTAGTCTGCTGACAGTAGTAAGTTGCAAAGTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT25380.27015404528539677No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25070.2668542913831717No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24240.25801946641914963No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG24080.25631636763090443No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT24070.2562099239566391No Hit
GTAGGAGACAGAGTCACCATCAATTGCCGGACAAGTCAGAGTGTTAGGAGCTATTTAAACTGGTATCAGCAGAAA22980.24460756346171858No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22640.24098847853669753No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCAATTGCCGGACAAGTCAGAGTGTTAGGAG22630.2408820348624322No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21930.2334309776638594No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21780.2318343225498795No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG21590.2298118927388383No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC21090.22448970902557205No Hit
GTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTCGATGGGAC20420.2173579828497952No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT19270.20511696030928273No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18960.20181720640705764No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT18420.19606924799673006No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18370.1955370296254034No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17980.19138572632905573No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17600.18734086670697334No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17240.1835088944334216No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16950.18042202787972716No Hit
CACCATCAGCAGTCTGCAACCTGAAGACTTTGCAACTTACTACTGTCAGCAGACTAACAGTATCCCGTGGACGTT16560.17627072458337945No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16410.1746740694693996No Hit
CCTGAAGACTTTGCAACTTACTACTGTCAGCAGACTAACAGTATCCCGTG16360.17414185109807295No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15880.1690325547333373No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15620.16626501920243886No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCAATTGCCGGACAAGTCAGA15490.1648812514369896No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15470.16466836408845895No Hit
ACTTACTACTGTCAGCAGACTAACAGTATCCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAGTCAAGCGAA15380.16371037102007102No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15340.16328459632300973No Hit
GTCTGCAACCTGAAGACTTTGCAACTTACTACTGTCAGCAGACTAACAGTATCCCGTGGACGTTCGGCCAGGGGA14950.15913329302666201No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT14810.15764308158694745No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGACTTCCACCTTGGTCCCCTGGCCGAACGTCCACGGGATA14750.1570044195413555No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC14740.15689797586709017No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14390.15317244726780377No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14310.15232089787368117No Hit
GTGTTAGGAGCTATTTAAACTGGTATCAGCAGAAACCAGGGAAAGCCCCTAGCCTCCTAATCTATCGTGCATCCA14280.15200156685088517No Hit
GTCCCAGATCCACTGCCACTGAACCTCGATGGGACCCCACTTTGTAAAGT14120.15029846806264No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTCG14120.15029846806264No Hit
GTAGTAAGTTGCAAAGTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA14060.149659806017048No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTCGATGGGACCCCA14000.14902114397145608No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13880.14774381988027216No Hit
GGCTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGTTTAAATAGCTCCTA13830.14721160150894552No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13780.14667938313761889No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGACTTTGCAACTTACTACTGTCAGCAGACTAACAGTATC13750.14636005211482292No Hit
ATTTAAACTGGTATCAGCAGAAACCAGGGAAAGCCCCTAGCCTCCTAATC13350.14210230514420988No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13220.14071853737876067No Hit
CCTAATCTATCGTGCATCCACTTTACAAAGTGGGGTCCCATCGAGGTTCAGTGGCAGTGGATCTGGGACAGATTT13160.14007987533316868No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC13130.13976054431037271No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12970.1380574455221275No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT12840.13667367775667827No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12830.13656723408241295No Hit
CTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCT12700.1351834663169637No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT12380.1317772687404733No Hit
GTGGGGTCCCATCGAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT12310.13103216302061602No Hit
ATAGATTAGGAGGCTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGTTTAAATAGCTCCTAACACTCTGACTTGT12230.13018061362649339No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12050.12826462748971754No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC11950.1272001907470643No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11770.1252842046102884No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11770.1252842046102884No Hit
CCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAGTCAAGCGAACTGTGG11720.12475198623896179No Hit
GCCACAGTTCGCTTGACTTCCACCTTGGTCCCCTGGCCGAACGTCCACGG11640.1239004368448392No Hit
GGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCC11510.12251666907938995No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11370.12102645763967539No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCA11200.11921691517716484No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11080.11793959108598094No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10950.11655582332053172No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT10770.11463983718375584No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGACTTTGCAACTTA10680.11368184411536791No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10500.11176585797859205No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10470.11144652695579606No Hit
CAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTA10360.11027564653887749No Hit
CCATCAGCAGTCTGCAACCTGAAGACTTTGCAACTTACTACTGTCAGCAG10350.11016920286461215No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC10250.10910476612195891No Hit
CCCCTAGCCTCCTAATCTATCGTGCATCCACTTTACAAAGTGGGGTCCCA10250.10910476612195891No Hit
CAGTATCCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAGTCAAGCGAACTGTGGCTGCACCATCTGTCTTCAT10020.10665656161385642No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10000.10644367426532576No Hit
GATTAGGAGGCTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGTTTAAAT9990.10633723059106043No Hit
GGACCAAGGTGGAAGTCAAGCGAACTGTGGCTGCACCATCTGTCTTCATC9950.10591145589399914No Hit
GTCTACTACTGTGTGAAAGATATCCAAAATGGAGTCGTACCACTTCTTAT9820.1045276881285499No Hit
CAGTAGTAAGTTGCAAAGTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA9810.10442124445428458No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG9750.1037825824086926No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9730.10356969506016196No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG9720.10346325138589664No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT9650.10271814566603936No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9570.10186659627191676No Hit
CTCCTAATCTATCGTGCATCCACTTTACAAAGTGGGGTCCCATCGAGGTT9530.10144082157485546No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9480.10090860320352882No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGACTTTGCAACTTACTACTGTCAGCAGA9460.10069571585499817No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCAATTGCCG9400.10005705380940623No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTATA504.387033E-665.05044670
CCTAGCG200.00645021353.5031265
GCTAGCG200.007545213751.42325220
GTGGTAT2300.040.1864241
GTATCAA12850.038.0957371
TCTAGAC400.002703289734.2164843
TATAGAC400.002703289734.2164843
TATCAAC14350.034.097262
TGTTAGA12300.033.3185270
AACCGTT1900.031.1941749
TCAACGC15800.030.9680824
ATCAACG16000.030.7948343
GTTTACG450.004689868530.57575832
AACGCAG16350.030.1356166
CAACGCA16250.030.1105045
AGAGTAC17100.028.83997311
GTCTACT2850.028.8276881
CGCGATT500.006767780528.36503462
ACAGCGT854.7010653E-628.1932858
TAAACCG2100.028.16830447
Produced by FastQC (version 0.11.5)