FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1054121
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTAGCCTGCTGACAATAGTAAGTTGCAAAATCTTCGGGCTGCAGGCTGCTGATGGTGAGAATGAAATCTGTC22720.21553502871112518No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20270.19229291513972305No Hit
GGCTAACACTTTCCCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCGCACGAACTGTGGCTGCACCAT17910.16990459349543363No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCG17470.16573049962954917No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15930.15112117109895354No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15920.15102630532927436No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC15850.15036224494152No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15070.142962714906543No Hit
GCCACAGTTCGTGCGATATCCACTTTGGTCCCAGGGCCGAAAGTGAATGG14700.13945268142841288No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14210.13480425871413243No Hit
GCATAGATCAGGAGCCTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC14190.13461452717477407No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14090.13366586947798212No Hit
GTGCAGCCACAGTTCGTGCGATATCCACTTTGGTCCCAGGGCCGAAAGTG13500.12806878906690977No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13410.12721499713979706No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT12960.12294603750423339No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12410.1177284201718778No Hit
GGGTATTAGCAGTTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAGGCTCCTGATCTATGCTGCATC12210.11583110477829395No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT12070.11450298400278525No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG11840.11232107130016383No Hit
GTCGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAGCAGTTGGTTAGCCTGGTATCAGCAGAAA11650.11051862167625918No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10940.10378315202903651No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15850.037.4324071
GTGGTAT3450.034.994321
TATCAAC18550.031.7860092
TCGTATG802.6810885E-630.30194540
ATCAACG20500.028.5942573
TCAACGC20700.028.151414
CAACGCA21250.027.4227855
AACGCAG21600.026.9784346
CTCGTAT906.727687E-626.9235239
TATATAC658.684052E-426.5240863
ACGCAGA23200.025.1178517
CCCTATA1101.1580833E-625.0773182
AGAGTAC23500.024.65046911
TACTAGA700.00133799124.6295092
CAGAGTA23300.024.56608610
GCAGAGT25000.023.3093669
CGCAGAG24900.023.2644988
TTAACGG750.001964709523.0564435
TTTAACG953.0218018E-421.83567434
GTACTAG953.0687515E-421.7859381
Produced by FastQC (version 0.11.5)