FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754934

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754934
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1054121
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTAGCCTGCTGACAATAGTAAGTTGCAAAATCTTCGGGCTGCAGGCTGCTGATGGTGAGAATGAAATCTGTC22230.21088660599684478No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20700.19637214323592833No Hit
GGCTAACACTTTCCCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCGCACGAACTGTGGCTGCACCAT18470.1752170765974684No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCG15380.14590355376659794No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15150.14372164106397653No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC14820.14059107066456317No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13980.13262234601151102No Hit
GTGCAGCCACAGTTCGTGCGATATCCACTTTGGTCCCAGGGCCGAAAGTG13830.13119935946632313No Hit
GCCACAGTTCGTGCGATATCCACTTTGGTCCCAGGGCCGAAAGTGAATGG13390.12702526560043867No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13280.12598174213396754No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13060.1238946952010253No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12590.11943600402610326No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12580.11934113825642409No Hit
GCATAGATCAGGAGCCTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC12380.11744382286284023No Hit
GGGTATTAGCAGTTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAGGCTCCTGATCTATGCTGCATC11990.11374405784535173No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT11880.1127005343788806No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10950.10387801779871571No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10920.10359342048967812No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10880.10321395741096137No Hit
GTCGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAGCAGTTGGTTAGCCTGGTATCAGCAGAAA10880.10321395741096137No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT10770.10217043394449025No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16850.037.9855771
TATCAAC18400.034.3969732
ATCAACG19950.031.7245273
CAACGCA20050.031.56635
AACGCAG20250.031.4243956
TCAACGC20250.031.2545344
GTGGTAT3250.030.7057931
AGAGTAC22600.028.01923411
ACGCAGA22900.027.7932227
CAGAGTA23100.027.5617410
CGCAGAG24400.026.2293518
GCAGAGT24450.026.0399279
TTAACGG951.0359938E-525.46714635
TAACCGT901.9740745E-423.2533953
AATCTGT11000.022.29194668
TAAGTTG10050.022.2732122
TAACGGC1103.2081924E-522.00589236
TCGGGCT10550.021.96538437
AATAGTA10500.021.6271117
GTAAGTT10550.021.5430121
Produced by FastQC (version 0.11.5)