FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences925165
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCGTCAGATCTCAGACTGGTCAGCTCCATGTAGGCTGTGCCGATGGACGTGTCCCTGGT30530.32999519004718075No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAGAGTGCTGCTGATTGTATATGAGCTG29390.3176730637237682No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAGAGTGCTGCTGATTGTATATGAGCTGCAGTAATAAT28670.30989066815108657No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC26530.2867596590878384No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG24770.2677360254657277No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT24260.2622234952684116No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT24050.2599536298930461No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC21960.2373630649667897No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT21950.23725497613939134No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21150.22860786994752286No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAAGTCTCCTGCAAGGCTTCTCAATACACCTTCAGCGA19820.21423205590354152No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19590.21174601287337935No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGATTATAACTATGTCTCCTGGTACCAGCAGCACCCAGGCAAAGC19080.2062334826760632No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18830.20353126199110427No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG18600.20104521896094207No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG18210.19682975469240624No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC17950.19401944518004896No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC17850.1929385569060654No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG17400.18807455967313938No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17010.18385909540460352No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG16770.18126496354704297No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC16480.17813038755249064No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGATTATAACTATGTCTCCTGGTACCAGCAGCA16240.17553625569493012No Hit
GTGTATTACTGTGCGAAAGATATAAAGAGAAATTACTATGATGTCGGGGA16090.17391492328395475No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG16000.17294212383736954No Hit
CTCTTAGGGTTGATCCAGCCCATCCACTCAAGCCCTTGTCCAGGGGCCTGTCGCACCCAGTGTAGATGGTAGTCG15860.17142888025379258No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15240.1647273729550945No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT15170.163970751163306No Hit
GTTATCGGCCCTCAAGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGC14570.15748542151940464No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC14380.15543173379883587No Hit
GCCTACATGGAGCTGACCAGTCTGAGATCTGACGACACGGCCGTGTATTA14270.15424275669745396No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG14200.15348613490566546No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14110.15251333545908027No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14020.15154053601249506No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC14000.15132435835769836No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT13880.15002729242891807No Hit
GATTATTACTGCAGCTCATATACAATCAGCAGCACTCTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT13570.14667653877956904No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG13480.14570373933298386No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC13280.14354196278501674No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13190.14256916333843153No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT13170.1423529856836348No Hit
CCCTAAGAGTGGTGGCACAAACTATGCACAGAAATTTCAGGGCAGGGTCACCATGACCAGGGACACGTCCATCGG13090.14148827506444797No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13050.14105591975485454No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG13040.1409478309274562No Hit
GTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGA13010.1406235644452611No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT12910.13954267617127755No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAGAGTGCTGCTGA12710.13738089962331043No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG12660.13684045548631865No Hit
GTGTAGATGGTAGTCGCTGAAGGTGTATTGAGAAGCCTTGCAGGAGACTTTCACTGAGGCCCCAGGCTTCTTCAC12350.13348970183696962No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT12320.13316543535477457No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT12200.13186836942599428No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12070.13046321466981564No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12030.13003085936022224No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11900.1286257046040436No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA11340.12257273026973568No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11330.1224646414423373No Hit
CATCTACACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGG11240.12149184199575212No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC11110.12008668723957347No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11060.1195462431025817No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10990.11878962131079321No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA10920.11803299951900473No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT10880.11760064420941128No Hit
GGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGCTGGATCAACCCTAAGAGTGGTGGCACAAACTATG10740.11608740062583431No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG10740.11608740062583431No Hit
CAGTAATACACGGCCGTGTCGTCAGATCTCAGACTGGTCAGCTCCATGTA10730.11597931179843596No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC10670.11533077883404581No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10650.1151146011792491No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT10520.11370944642307049No Hit
GTCCTCTAGAGAATCCCCTGAGAGCTCCGTTCCTCACCATGGACTGGACCTGGAGGATCCTCTTCTTGGTGTCAG10500.11349326876827377No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA10490.11338517994087542No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC10080.10895353801754282No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10080.10895353801754282No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10070.10884544919014447No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT9930.10733220560656748No Hit
CCATGTAGGCTGTGCCGATGGACGTGTCCCTGGTCATGGTGACCCTGCCCTGAAATTTCTGTGCATAGTTTGTGC9890.10689985029697406No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC9830.10625131733258392No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGA9830.10625131733258392No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC9760.10549469554079544No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGCTGGATCAACCCTAAGAGTGGT9720.10506234023120199No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA9720.10506234023120199No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC9670.10452189609421023No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT9670.10452189609421023No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC9660.10441380726681188No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9580.10354909664762502No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT9570.10344100782022667No Hit
GCTTGGTCCCTCCGCCGAATACCAGAGTGCTGCTGATTGTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAG9480.10246820837364146No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG9420.10181967540925134No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC9410.10171158658185296No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC9390.10149540892705625No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC9350.10106305361746283No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC9300.10052260948047104No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTAA150.002163964370.4906966
CCTATAT200.00736642851.736051
TGTAAAG400.00874004249.5422170
GGTATCA5650.046.3945731
GTATCAA14100.040.850691
AGGGGGG1352.3284883E-839.14446670
TATCAAC15600.037.129782
GTGGTAT1500.036.790081
TCAACGC16750.034.580574
AACGCAG16850.034.579966
ATCAACG16800.034.477653
TGGTATC1500.034.477652
CAACGCA17050.034.1743285
GTAATAC7450.032.4073681
TCGTATG751.620052E-632.3223940
CCTAGAC553.2689385E-431.3433173
TAATACA7750.031.1411042
CGCAGAG18700.030.97298
CTCCTAG450.004628328630.6584011
CTAGACT450.004636904730.6468014
Produced by FastQC (version 0.11.5)