FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238651
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT6550.27445935696896306No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG1350.076.8415370
TATCACG150.002363596868.923745
CAATTTT200.007250447851.9321735
TTATACA1400.051.6928062
CTTGTCA200.007384144751.6928022
CCTTATA200.007384144751.6928022
CTTATAG200.007384144751.6928023
ATACAGG200.007384144751.6928026
GGCTATA200.007384144751.6928022
CTTATAC1450.049.9207531
TATACAC1450.049.9102943
GTATCAA12600.043.4967081
CTCGTAT1700.042.8669239
ATACACA1700.042.5705454
GTGGTAT1550.042.2524341
GGTATCA4550.041.6659361
TACACAT1750.041.3542445
TGGTATC1600.040.923472
TCGTATG1800.040.50251440
TATCAAC13850.039.562722
Produced by FastQC (version 0.11.5)