FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754940

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754940
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238651
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13830.5795073140275967No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACTT150.002316792369.27212534
CGACAAT150.002361667668.93790414
CCCTGTA200.00740264851.6600847
ACCTTAC200.007408786651.6492581
CCAATAC200.007414928651.6384353
TTCCGTA200.007414928651.6384352
GGTATCA4150.046.4635851
GTATCAA12550.044.99591
GTGGTAT1200.043.0410461
TATCAAC14500.038.9365652
CCCTACA400.002619810634.4256252
TGGTATC1500.034.425622
AACGCAG16650.034.1154826
CAGAGTA16050.033.71729310
AGAGTAC16100.033.61258311
CAACGCA17100.033.2177055
GCAGAGT16900.032.8372889
ATCAACG17300.032.6346933
ACGCAGA17600.032.091877
TCAACGC17750.031.8073354
Produced by FastQC (version 0.11.5)