FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754942

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754942
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1095707
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC29360.26795484559284555No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20400.1861811597443477No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG20210.1844471195310425No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18220.16628532992853015No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17730.16181333148369045No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGAGACCCCCGGGCAGTGGGTCACCATCTC16350.14921872361863162No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT13270.1211090191082105No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGAGACCCCCGGGCAGTGGGTCAC12830.1170933470352932No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG12800.11681955121213974No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12730.11618069429144835No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12670.11563310264514144No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG12580.11481171517568109No Hit
GCCTTGGGCTGACCTGGGAGCTGCTGGTAGCAGTCTACAGAGTTATGTCC12500.11408159298060522No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12450.1136252666086828No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11630.1061415141091551No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11460.10459000444461887No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT11210.10230837258500675No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTA601.49560165E-552.893270
GTATCAA23650.041.5788841
GGTATCA9150.041.1901781
TATCAAC26350.037.1731222
ATCAACG28250.034.4289473
TCAACGC28500.034.2471054
CAACGCA28800.033.890365
AACGCAG29750.032.8081476
ACGCAGA31950.030.5546347
AGAGTAC32750.029.82868411
CAGAGTA33150.029.15856410
CGCAGAG34150.028.6983458
GCAGAGT34250.028.422249
GTGGTAT3350.026.5919761
AGTACGG27050.026.10360313
GAGTACG27250.025.78623212
TGGTATC3200.025.6864992
TACGGGA11800.023.81839815
GAGTACT11850.023.42972612
GTACGGG30350.022.92650814
Produced by FastQC (version 0.11.5)