FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1199407
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCCCAGCACTGAGGCTGCTATCCCATGCT28580.23828441888366503No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC27830.23203132881498942No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCCCAGCACTGAGG26480.22077576669137333No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20930.17450290018317385No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG20330.16950042812823338No Hit
GGACAGAAGGTCACCATCTCCTGCTCTGGAAGCAACTCCAACATTGGTCTTCATTATGTATCCTGGTACCAGCAG18600.15507663370315497No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC18230.15199177593594168No Hit
CCCCAGCACTGAGGCTGCTATCCCATGCTCCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGC17950.14965728897696945No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG17870.1489902927029774No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC17500.14590543493576408No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17490.1458220604015151No Hit
ATCCCATGCTCCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGG16930.14115308648357064No Hit
GTGCTGGGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC15980.13323250572991485No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG15540.1295640262229585No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15100.12589554671600217No Hit
CCCAGGAACAGCCCCCAAACTCCTCATTTATGACAATAATCGGCGACCCTCAGGGATTCCTGACCGATTCTCTGC14650.12214369267479679No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCCAGGGTGG14340.11955908211307754No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14320.11939233304457952No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG14240.11872533677058747No Hit
GCATGGGATAGCAGCCTCAGTGCTGGGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG14150.11797496596234638No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13530.11280574483890789No Hit
GTCTGGAGTCCGGTGATGCCCAGGGTGGCTGACGTGCCAGACTTGGAGGC13210.11013775974293963No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13000.10838689452371048No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12990.10830351998946146No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT12530.10446829141400708No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12110.10096656097554875No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8950.043.0878141
GTATCAA24450.042.670061
TATCAAC27200.038.4601172
GTGGTAT3250.036.020941
TGGTATC3250.034.9410972
ATCAACG29950.034.9287223
TCAACGC30050.034.812494
CAACGCA30700.034.1875085
AACGCAG31350.033.4786766
AGAGTAC33950.030.51187311
CGCAGAG34650.030.3908068
ACGCAGA34650.030.2902287
CAGAGTA34800.029.76661310
GCAGAGT36100.028.9806779
TTAACGG756.129419E-527.62262235
TATAAGA3400.026.3148172
GAGTACT10400.024.81818612
GTAGTTT1801.3830395E-524.53306470
CAATAAG3351.6370905E-1124.48075370
AGTACGG32400.024.11143113
Produced by FastQC (version 0.11.5)