FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1199407
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC27680.23078071080125429No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCCCAGCACTGAGGCTGCTATCCCATGCT23360.19476291200568283No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGCGGCCACAGGACAGAAGGTCACCATCTCCTG23220.1935956685261967No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20380.16991730079947842No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19910.16599869768977502No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGCGGCCACAGGACAGAAGGTCAC18730.15616050264839207No Hit
GGACAGAAGGTCACCATCTCCTGCTCTGGAAGCAACTCCAACATTGGTCTTCATTATGTATCCTGGTACCAGCAG18180.15157490326469664No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCCCAGCACTGAGG18020.1502409107167125No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16740.13956897033283946No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG16500.13756798151086327No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC16270.13565036722313611No Hit
GTGCTGGGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC16260.13556699268888708No Hit
ATCCCATGCTCCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGG15020.1252285504420101No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC14370.11980920571582458No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG14360.11972583118157556No Hit
CCCAGGAACAGCCCCCAAACTCCTCATTTATGACAATAATCGGCGACCCTCAGGGATTCCTGACCGATTCTCTGC14350.11964245664732656No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13990.11664097341436226No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG13960.11639084981161524No Hit
CCCCAGCACTGAGGCTGCTATCCCATGCTCCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGC13880.11572385353762317No Hit
GCATGGGATAGCAGCCTCAGTGCTGGGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG13430.1119719994964178No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT13150.1096375125374456No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12900.10755314918122039No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12570.10480178955100312No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT12290.10246730259203089No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12040.1003829392358057No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12010.10013281563305866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24850.042.1500241
GGTATCA9800.041.350461
GTGGTAT3700.038.9827081
TATCAAC27100.038.5157362
TGGTATC3700.037.118652
TCAACGC29250.035.4498984
ATCAACG29450.035.3257333
CAACGCA29800.034.9108355
AACGCAG30300.034.336186
AGAGTAC32800.031.74423811
CAGAGTA33250.031.10792210
CGCAGAG33800.030.797358
ACGCAGA34300.030.337027
GCAGAGT35000.029.5525259
CGCATAC500.00701210428.16042361
GTGTATA1608.367351E-1125.7564321
AGTACGG33400.024.07495713
GAGTACG34100.023.78229512
GTATAAG3350.023.5780261
GTACTTT9550.023.38870214
Produced by FastQC (version 0.11.5)