FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences678716
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC2250.042.6908233
GTATCAA29200.037.6168331
GGTATCA12900.037.252331
TTATACA2150.036.698512
CTTATAC2200.035.88561
CTCGTAT1750.035.6185539
AGACGTA2250.035.1114425
TCGTATG2100.034.6626640
ATATAAC501.9235871E-434.3051263
CGTATGC2250.033.92773441
GTGGTAT4750.033.24141
TGGTATC4800.032.8757482
TATCAAC33500.032.871482
AGGGGGG3150.032.08181870
TACACAT2700.031.7640085
TATTAGA1001.5301339E-830.8746152
AACGCAG36150.030.5567136
CAACGCA36350.030.3885865
ATCAACG36450.030.21113
ACGAGAC2650.029.78523422
Produced by FastQC (version 0.11.5)