FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences678716
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14720.21688010891153295No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAACG405.2155196E-542.817453
GTATCAA31550.038.7767751
GGTATCA13600.036.78891
TATCAAC35400.034.4474872
GTGGTAT5400.033.6344951
AACGCAG38250.031.7912566
ATCAACG38650.031.4622423
CAACGCA38400.031.397155
TCAACGC38850.031.121644
AGAGTAC38500.030.90264711
CAGAGTA38650.030.69400610
ACGCAGA39950.030.530937
CGCAGAG40150.030.2304538
TGGTATC5900.030.1899322
GCAGAGT40700.029.9061769
CGCTAGA500.007535309527.74660340
AGTACGG39250.026.5558913
GAGTACG39950.026.17640512
ACGGGTA1759.094947E-1225.4777116
TACGGGC9200.023.48246615
Produced by FastQC (version 0.11.5)