FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences502462
Sequences flagged as poor quality0
Sequence length20-76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT11190.2227034084169549No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTTA200.00752010151.46312726
AATAACG200.0075646551.3863075
AGGGGGG2400.050.71351670
TTATACA1700.050.378732
CTTATAC1750.048.96371
GTAACAC352.3677503E-548.939343
GTGGTAT3550.045.377631
GGTATCA11350.043.182971
GTATATA405.195085E-542.843241
GTATCAA29200.040.8477971
TATACAC2200.040.4861833
TGGTATC4000.040.2526052
CGATATT350.001335591139.49735336
TACACAT2250.038.063935
TCTTATA557.752258E-637.371862
ATACACA2350.036.4441874
TATCAAC33050.035.9678232
ACTTGTA501.6183304E-435.32621861
CTCGTAT2400.034.6820539
TCGTATG2450.032.59148840
Produced by FastQC (version 0.11.5)