FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences502462
Sequences flagged as poor quality0
Sequence length20-76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23360.46491077932261543No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5900.045.8237421
TGGTATC5900.045.8100622
GTATCAA34100.038.8393631
GGTATCA14300.037.573281
TATCAAC37650.035.3484572
TACGGAC501.9467957E-434.23302515
AACGCAG39750.033.2194756
CAACGCA41500.031.9010875
ATCAACG41600.031.827573
CAGAGTA41200.031.4973310
AGAGTAC41350.031.38307211
TCAACGC42800.030.9321294
GCAGAGT42750.030.7557879
ACGCAGA43350.030.712797
CGCAGAG43050.030.6944188
CTAAGGA450.00481322130.4111823
TAAGAGC450.004815564530.4081576
TACTGCT854.704427E-628.1862877
AGTACGG43250.025.88769513
GAGTACG43650.025.72890712
Produced by FastQC (version 0.11.5)