FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1055992
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT17510.1658156501185615No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG6350.041.7373370
GTATCAA34700.041.2908061
GGTATCA13000.041.1676641
TATCAAC39150.036.9155582
CTTATAC4800.035.283471
TCAACGC42200.034.40794
CGTATGC4400.033.9089341
ATCAACG42750.033.8068773
CAACGCA43250.033.4160465
AACGCAG44400.032.550546
CTCGTAT4350.031.7746639
TCTCGTA4250.030.84697338
AGACTCG5150.030.27767425
CGCAGAG47950.029.8597138
ACGCAGA48900.029.4180557
TCGTATG4850.029.27507840
GACGTCA5150.029.12561831
ACTCGAC5250.029.10496727
TTAACGG1302.1100277E-1029.02412435
TATGCCG5050.028.9698443
Produced by FastQC (version 0.11.5)