FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1055992
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42080.3984878673323282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13600.039.4877851
GTATCAA33600.037.7966351
TATCAAC38100.033.3135262
TCAACGC39500.031.9588474
ATCAACG39800.031.8875683
CAACGCA41050.030.8343435
TGGTATC5300.030.5719452
AACGCAG42600.029.87096
GTGGTAT5400.028.7719061
CGCAGAG45850.027.7653858
AGAGTAC45200.027.64433511
ACGCAGA46450.027.4002487
TACCGAT757.14384E-527.0077767
ATACCGA757.153057E-527.002666
CAGAGTA46600.026.88628810
GCAGAGT48650.025.892199
GAGTACG30100.024.45867212
TCTGTCG1801.6370905E-1124.3877948
AGTACGG30850.023.97351813
TATACTG2700.023.752345
Produced by FastQC (version 0.11.5)