FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1192908
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31140.2610427627277208No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAAGTA27090.22709211439608085No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT23480.19682993156219927No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22430.18802791162436666No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG21170.17746548769896756No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT20080.1683281527158842No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20030.16790900890932076No Hit
GTAATAGACGGCCGTGTCCGCGGCGGTCACAGAACTCACCTTCAGGGAGA19580.16413671465024965No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19410.16271162570793388No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18710.1568436124160455No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTGGCCTGGTACCAACAGAAACTTGGCCAGGCTCCCAGGCT18210.15265217435041092No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17960.15055645531759365No Hit
GTGCAGCCACAGTTCGTTTGACATCCACTTTGGTCCCAGGGCCGAAAGTGATACGAGGCCAGTTGCTACGCTG17640.14787393495558754No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16720.14016168891481992No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAAC16550.13873659997250418No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16100.13496430571343307No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15200.12741971719529085No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT14870.12465336807197201No Hit
GCGTAGCAACTGGCCTCGTATCACTTTCGGCCCTGGGACCAAAGTGGATGTCAAACGAACTGTGGCTGCACCAT14000.11736026583776787No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT13950.11694112203120442No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAACTGG13910.11660580698595366No Hit
GTCTATTACTGTGCGAGAGATCTTGACGACTACGGTGACCCGCTTTCTCG13310.11157608130719218No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13270.11124076626194142No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13090.10973184855831296No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13050.1093965335130622No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT12630.10587572553792915No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACATCCACTTTGGTCC12600.10562423925399109No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTGGCCTGGTA12340.10344469145986111No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12190.10218726004017073No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11960.10025919852997883No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTAA200.00744091351.605491
GGTATCA9350.041.946711
GTATCAA23100.039.7652661
TATCAAC26450.034.461472
GTGGTAT3300.034.4036641
TACTGAT7600.033.90583870
ATCAACG27350.033.453223
TCAACGC28050.032.4957544
CAACGCA28300.032.330235
AACGCAG28700.031.9994856
ACGCAGA30900.029.833777
AGAGTAC30700.029.35708811
CGCAGAG32050.028.7632928
TGGTATC3800.028.060262
GCAGAGT33150.027.810029
TCGTATG1004.839385E-727.71464240
CAGAGTA33150.027.0836410
TGTTAGA5300.026.0886270
TAGACGG5700.024.7413065
TAATAGA5700.024.137862
Produced by FastQC (version 0.11.5)