FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1192908
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31840.2669107760196092No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAAGTA24700.2070570404423476No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT22590.18936917180536972No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20670.17327404963333298No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20160.16899878280638575No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG20080.1683281527158842No Hit
GTAATAGACGGCCGTGTCCGCGGCGGTCACAGAACTCACCTTCAGGGAGA18830.1578495575517978No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT18170.15231685930516017No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17760.14887988009133982No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17380.14569438716145755No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTGGCCTGGTACCAACAGAAACTTGGCCAGGCTCCCAGGCT17080.1431795243220768No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17000.14250889423157526No Hit
GTGCAGCCACAGTTCGTTTGACATCCACTTTGGTCCCAGGGCCGAAAGTGATACGAGGCCAGTTGCTACGCTG16930.14192209290238644No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16790.14074849024400876No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15810.132533271635365No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAAC15300.12825800480841776No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14980.12557548444641162No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT14540.12188701894865321No Hit
GTCTATTACTGTGCGAGAGATCTTGACGACTACGGTGACCCGCTTTCTCG13880.11635432070201557No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT13650.11442625919182367No Hit
GCGTAGCAACTGGCCTCGTATCACTTTCGGCCCTGGGACCAAAGTGGATGTCAAACGAACTGTGGCTGCACCAT13620.11417477290788561No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13510.113252656533446No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAACTGG13290.11140842378456678No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT13200.11065396493275256No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTGGCCTGGTA12910.10822293085468453No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12600.10562423925399109No Hit
CTGCTACTCTGGCTCCCAGAAAGTACCGGAGAAATTGTGTTGACACAGTC12340.10344469145986111No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20250.041.375661
TACTGAT7950.040.6686670
GGTATCA8150.037.9197121
TATCAAC23350.035.4220432
GTGGTAT3300.035.3789671
ATCAACG24800.033.487983
CAACGCA24700.033.4846155
TAACCCG500.003058991633.36450669
TCAACGC25200.032.956424
AACGCAG25900.031.9345446
AGAGTAC27200.030.05493211
ACGCAGA27650.029.919647
ATAGACG6550.029.34084
CGCAGAG28350.029.1882278
GTCTACT6300.029.04639667
AATAGAC6550.028.8168563
TTGTCTA6500.028.26739365
TGTCTAC6550.028.1915266
GCAGAGT29400.028.0372319
CAGAGTA29200.027.8787410
Produced by FastQC (version 0.11.5)