FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005754955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences901862
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCACTACGGAAACTCAATGTGGACGTTCGGCCAAGGGA26580.2947235829871976No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23590.2615699519438673No Hit
CAGTAATACACTGCAAAATCCTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA20830.2309666002115623No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA18850.20901202179490877No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14740.1634396393239764No Hit
GTCTGGGACAGACTTCACTCTCACCATCACCAGACTGGAGCCTGAGGATTTTGCAGTGTATTACTGTCAGCACTA14690.1628852307781013No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14600.16188729539552615No Hit
CTCCTGCAGGGCCAGTCAGAGAATTAGTAGCAGCTACTTAGCCTGGTACC14460.16033495146707588No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14250.15800643557440053No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT13570.1504664793504993No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13090.14514415731009844No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC12760.14148506090732285No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12670.14048712552474768No Hit
GTGTATTACTGTCAGCACTACGGAAACTCAATGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA12610.1398218352696976No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12090.13405598639259664No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11490.12740308384209556No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10920.12108282641911955No Hit
ATACATGAGGAGCCTGGGAGCCTGGCCAGGTCTCTTCTGGTACCAGGCTAAGTAGCTGCTACTAATTCTCTGA10770.1194196007814943No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGAATTAGTAG10720.1188651922356192No Hit
CCTCATGTATAGTTCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTT10340.11465168728696852No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC10230.11343198848604331No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC9970.11054906404749285No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9560.10600291397131713No Hit
GTAATACACTGCAAAATCCTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA9480.10511586029791697No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9260.10267646269606658No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)