FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences901862
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCACTACGGAAACTCAATGTGGACGTTCGGCCAAGGGA25430.28197218643207056No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23120.25635851161264145No Hit
CAGTAATACACTGCAAAATCCTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA18550.20568557051965825No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA18370.2036896997545079No Hit
GTCTGGGACAGACTTCACTCTCACCATCACCAGACTGGAGCCTGAGGATTTTGCAGTGTATTACTGTCAGCACTA14500.16077847830377598No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13940.15456910258997497No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13700.15190794156977452No Hit
CTCCTGCAGGGCCAGTCAGAGAATTAGTAGCAGCTACTTAGCCTGGTACC13470.14935766225874914No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT13240.14680738294772372No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC12560.13926742672382247No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12290.13627362057609702No Hit
GTGTATTACTGTCAGCACTACGGAAACTCAATGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA12160.13483215835682177No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11990.13294716930084646No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11800.13084041682652112No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11580.12840101922467073No Hit
ATACATGAGGAGCCTGGGAGCCTGGCCAGGTCTCTTCTGGTACCAGGCTAAGTAGCTGCTACTAATTCTCTGA11140.12352222402096996No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10670.1183107836897441No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9790.10855319328234253No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGAATTAGTAG9780.1084423115731675No Hit
GTAATACACTGCAAAATCCTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA9640.10688996764471727No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9460.10489409687956695No Hit
CCTCATGTATAGTTCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTT9310.10323087124194166No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC9120.10112411876761634No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGTA200.006993087552.42090658
GTATCAA15450.040.5785061
TATCAAC17300.036.2211042
TGTTAGA3650.035.3025370
GTATAGA501.8779458E-434.447141
ATCAACG18600.033.689523
CAACGCA18850.033.4253585
TCAACGC18750.033.4200064
GGTATCA6350.033.0909541
AACGCAG19350.032.3837286
ACTAAGC450.00466835330.6043973
CGCAGAG21450.029.0576118
ACGCAGA21450.029.0567
AGCGTAG1153.6280107E-728.64021969
GTGGTAT5150.028.0928121
GCAGAGT22300.027.9624399
AACTGTG10950.027.65364870
TGTATAA1005.105576E-727.543962
CAGAGTA23200.026.5821710
AGAGTAC23550.026.0408111
Produced by FastQC (version 0.11.5)