FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754957

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754957
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences734815
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCTATACTGCGTCCAGTTTGCAAAGTGGGGTCCCATCGAGGTTCAGTGGCAGTGGATCTGGGACAGAATTCA27640.37614909875274727No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23650.3218497172757769No Hit
GTATAGATGAGGAGTTTAGGGGCTTTTCCCGGTTTCTGCTGATACCAATT19760.2689112225526153No Hit
TCTATACTGCGTCCAGTTTGCAAAGTGGGGTCCCATCGAGGTTCAGTGGCAGTGGATCTGGGACAGAATTCACT19130.26033763600362No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16930.23039812741982674No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16560.22536284643073426No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14610.19882555473146302No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14200.193245919040847No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13930.1895715248055633No Hit
GAGTTACAGTACCTCTACGACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT13510.18385580043956642No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13220.17990922885352095No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13100.17827616474895042No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12420.1690221348230507No Hit
CACCATCACCAGTCTGCAACCTGAAGATTTTGCATCTTACTACTGTCAACAGAGTTACAGTACCTCTACGACTTT12290.16725298204309927No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11710.15935983887100835No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11280.15350802582963058No Hit
CAGTATAGATGAGGAGTTTAGGGGCTTTTCCCGGTTTCTGCTGATACCAATTTAAATAGTTCCTAATGTTCTGA11230.1528275824527262No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11120.15133060702353654No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10950.1490170995420616No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTC10420.1418043997468751No Hit
TCTTACTACTGTCAACAGAGTTACAGTACCTCTACGACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA10410.1416683110714942No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9370.12751508883188284No Hit
GAATTCACTCTCACCATCACCAGTCTGCAACCTGAAGATTTTGCATCTTACTACTGTCAACAGAGTTACAGTAC8800.1197580343351728No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8790.11962194565979192No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8560.11649190612603173No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8360.11377013261841416No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7730.10519654606941883No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7650.1041078366663718No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7650.1041078366663718No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7520.10233868388642038No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG7500.10206650653565863No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG7370.10029735375570721No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12050.036.3941841
CGTATGC1250.035.99244741
TCGTATG1350.035.84306740
GCCTAAT952.5127342E-432.7753770
TATCAAC13550.032.0906032
GTGGTAT2250.031.9775981
TAACGGC652.348174E-531.79071236
TTTAACG652.3636005E-531.76027934
TAGTTCG450.004158145331.33608857
TTAACGG553.2959183E-431.29832535
ATCAACG14050.030.9485863
TCAACGC14650.029.6810674
CAACGCA14800.029.3802495
TAACCGT604.929501E-429.21994853
CTCGTAT1301.9463187E-1029.2174839
ACCGTAC1650.029.09828427
AACGCAG14950.029.0854646
TTAGACG605.393008E-428.7747939
CGCACTA605.6132267E-428.5786727
ATCTATA6200.027.6116122
Produced by FastQC (version 0.11.5)