FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences734815
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCTATACTGCGTCCAGTTTGCAAAGTGGGGTCCCATCGAGGTTCAGTGGCAGTGGATCTGGGACAGAATTCA27130.3692085763083225No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22420.3051108102039289No Hit
GTATAGATGAGGAGTTTAGGGGCTTTTCCCGGTTTCTGCTGATACCAATT18870.25679933044371717No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17420.2370664725134898No Hit
TCTATACTGCGTCCAGTTTGCAAAGTGGGGTCCCATCGAGGTTCAGTGGCAGTGGATCTGGGACAGAATTCACT17250.23475296503201484No Hit
GAGTTACAGTACCTCTACGACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT14210.19338200771622788No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13440.1829031797119003No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13340.1815422929580915No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13320.18127011560732975No Hit
CACCATCACCAGTCTGCAACCTGAAGATTTTGCATCTTACTACTGTCAACAGAGTTACAGTACCTCTACGACTTT13240.18018140620428272No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13150.17895660812585482No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13020.17718745534590338No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12620.17174390833066827No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12060.1641229425093391No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11940.16248987840476856No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10930.14874492219129984No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10560.14370964120220736No Hit
CAGTATAGATGAGGAGTTTAGGGGCTTTTCCCGGTTTCTGCTGATACCAATTTAAATAGTTCCTAATGTTCTGA10290.14003524696692365No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9960.13554432067935468No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9890.13459169995168851No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTC9860.13418343392554588No Hit
TCTTACTACTGTCAACAGAGTTACAGTACCTCTACGACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA9840.13391125657478414No Hit
GAATTCACTCTCACCATCACCAGTCTGCAACCTGAAGATTTTGCATCTTACTACTGTCAACAGAGTTACAGTAC9420.12819553220878724No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9340.1271068228057402No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8790.11962194565979192No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8500.11567537407374646No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8090.11009573838313044No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7550.10274694991256302No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12600.041.2411271
TGACTGG8000.035.43715370
TATCAAC14700.035.342322
ATCAACG14800.034.4106833
TCAACGC15450.032.962994
CAACGCA15550.032.751015
AACGCAG15800.032.2327966
AGAGTAC15950.031.30169711
ACGCAGA16600.030.687767
CGCAGAG17450.028.8069748
GCAGAGT18250.027.7315759
GTGTATA1405.2023097E-1026.8609941
TGTTAGA6750.026.72700970
ATCTATA8250.026.5151942
GTGGTAT2200.026.4170111
GATACTA659.138391E-426.292112
CAGAGTA18900.026.2350510
AGTACGG15150.025.28023313
GAGTACG15250.025.11446212
CATCTAT8950.024.8283461
Produced by FastQC (version 0.11.5)