FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences433400
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT6430.14836179049377018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTATA551.5458681E-743.61726413
CTCGTAT901.8189894E-1242.3160339
TCGTATG1000.041.59026740
GGTATCA8050.039.6105271
TAGAGTG451.0406002E-438.0785645
GTATCAA18700.037.220141
ATCTCCG1300.034.2707110
TATCCCG400.002680795334.270715
GTGGTAT2850.033.6850131
AGGGGGG1855.2568794E-1033.45253470
CGTATGC1350.033.40999241
TATCAAC21350.032.745782
TATGCCG1400.032.2884343
TGGTATC3000.031.9859942
ATCAACG23050.030.4793743
TCAACGC23150.030.347714
AACGCAG23250.030.0697826
TGTATAA802.9034618E-629.9868714
CAACGCA23500.029.8957255
AGAGTAC23750.028.71524811
Produced by FastQC (version 0.11.5)