FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences799377
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATACG306.070771E-446.3625846
CGCTAAT955.456968E-1239.94914632
CTCGCTA801.6589183E-938.76803630
GTATCAA14400.038.7243771
GGTATCA6250.036.349281
CGGTTAT400.00253440634.66894541
TCGTATG904.962203E-934.65584640
TATCAAC16150.034.5195962
GTATACG400.002624960234.4216651
GCCTAAT1607.226845E-933.34042770
CGTATGC958.380084E-932.8442641
TCGCTAA958.8384695E-932.6651831
ATCAACG17250.032.517843
TCAACGC17400.032.2375144
CGACCGG1301.7858856E-631.91562370
CAACGCA17850.031.6175985
CGTTAAA450.00467925830.58938417
AACGCAG18750.030.099956
ACGACCG1004.3247383E-628.49523269
TAGAACG500.00782934227.5304434
Produced by FastQC (version 0.11.5)