FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences799377
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9160.11458923636782144No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16500.038.077491
GTGGTAT3700.033.404271
GGTATCA8900.032.7891461
TATCAAC19050.032.7797662
ATCAACG20050.031.1448673
TCAACGC20400.030.7787084
CAACGCA21450.029.5919695
CCCTATA1052.4570909E-829.4091632
AACGCAG21750.029.3415576
AGAGTAC23000.027.63056611
CAGAGTA23300.027.42224110
ACGCAGA23800.026.8293427
CGCAGAG24450.026.4018618
AGTACGG19050.025.24520713
GAGTACG19300.024.91819812
GCAGAGT26250.024.8639559
TGGTATC4550.024.1305962
TACGGGA7900.021.74146715
GTACGGG21900.021.6461614
TTCGTAT1450.003164978221.2357270
Produced by FastQC (version 0.11.5)