FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences636789
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2250.041.5186270
GTATCAA22100.039.4634131
GTGGTAT3800.038.854211
GGTATCA9950.037.2695271
TGGTATC4050.034.743812
TATACAC1900.034.3201033
TATCAAC26150.033.4670222
TCGTATG1450.033.4598240
CTTATAC1850.033.408271
CTCGTAT1350.033.3237439
ATCAACG28650.030.5466863
CAACGCA28800.030.387595
TTATACA2050.030.1347242
AACGCAG29350.029.9350816
TCAACGC29750.029.532594
ACGCAGA30750.028.4605737
AGAGTAC30350.028.38334811
CAGAGTA31000.027.78821210
CGCAGAG31750.027.5641778
GCAGAGT32500.027.0336789
Produced by FastQC (version 0.11.5)