FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1059164
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14750.13926077547952914No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC13370.12623163173974947No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12970.12245506833691477No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12690.1198114739549305No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12110.1143354570208202No Hit
GTCCGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA11700.11046447953291463No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC11470.10829295557628468No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAATCACACCACTCAGGCTGCTATCCCATGTT11260.10631025978979648No Hit
GTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCCTCCACCAAGGGCCCATCGGTCTTCCCC10710.10111748511089878No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC10690.10092865694075705No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9450.043.296611
GTATCAA24150.039.5791471
TATCAAC24900.038.0910452
TCGTATG1750.037.59604640
ATCAACG26450.035.8588683
CGTATGC1850.035.59430741
AGGGGGG3250.035.56492670
TCAACGC27150.035.0609054
CAACGCA27150.034.9343345
TATGCCG1850.033.78687343
AACGCAG28450.033.338046
CTTATAC2500.031.6319961
AGAGTAC30000.031.61556811
CGCAGAG30750.030.7327068
TTAAGCG450.004601171230.695736
ACGCAGA31550.030.0623477
CAGAGTA31600.029.90603310
CACGAGA2350.029.25073821
AGTACGG23300.028.02275813
CGAGACT2350.027.78820223
Produced by FastQC (version 0.11.5)