FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1135366
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTATACAGCTTCAACAACAAGAACTACTTAGCTTGGTACCAGCAGAG84330.7427560804181207No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT74340.6547668328979378No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC60420.5321631967136589No Hit
GTATAAGACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA51810.4563286200220898No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC50230.4424124026965754No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG49580.4366873765816486No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGAG49180.4331642835878474No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC48340.42576578830086514No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC47100.41484420002008165No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT44930.3957314205287106No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43550.3835767497000967No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT43540.3834886723752517No Hit
ACTTAGGAGGCTGGCCTGGCCTCTGCTGGTACCAAGCTAAGTAGTTCTTGTTGTTGAAGCTGTATAAGACACT42180.3715101561963279No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCTCAGACCCGCTGCCA40070.352925840654027No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGAGACAGACTTCACTCTCAC39910.35151660345650654No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT38120.3357507623092465No Hit
GTCTCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGTATTCCCGGGT37970.33442960243657116No Hit
GTACCAGCAGAGGCCAGGCCAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAATACGGGGTCCCTGA36900.3250053286781531No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG36450.3210418490601269No Hit
CTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAATACGGGGTCCCT34440.3033383067662762No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG33440.2945305742817734No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC32530.28651553772087596No Hit
GTCTGAGACAGACTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG31780.2799097383574988No Hit
CTGCTGGTACCAAGCTAAGTAGTTCTTGTTGTTGAAGCTGTATAAGACAC31320.27585818141462753No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30880.2719827791214463No Hit
GTCAGGGACCCCGTATTCCCGGGTAGATGCCCAGTAAATGAGCAACTTAGGAGGCTGGCCTGGCCTCTGCTGGTA30650.26995700065001066No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG30220.26616967568167443No Hit
GTTTATTACTGTCAACAATATTACAAAACTCCGCTCTCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA30060.26476043848415404No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT29750.26203004141395814No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT29490.2597400309679874No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29380.2587711803946921No Hit
GGCCAGGCCAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAA28760.25331038625430036No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG28750.25322230892945535No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28470.2507561438337946No Hit
GAGTGAAGTCTGTCTCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG27520.24238879797351692No Hit
ATTCAGTGGCAGCGGGTCTGAGACAGACTTCACTCTCACCATCAGCAGCC26600.23428568408777434No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26040.22935335389645278No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTCTTATA25710.22644680217656687No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA25500.22459717835482126No Hit
GACTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTACAAAACT25420.22389255975606104No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC25010.2202813894374149No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA24540.2161417551696986No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCTCAGACCCGCTGCCACTGAATCGGTCAGGGACC24190.2130590488001226No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC22840.2011686099460438No Hit
GGCCTGGCCTCTGCTGGTACCAAGCTAAGTAGTTCTTGTTGTTGAAGCTG22570.19879052217522808No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG22570.19879052217522808No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22340.19676474370379243No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22150.19509127453173689No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTACAAAACTCCGC21950.19332972803483636No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA21900.1928893414106112No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA21620.19042317631495043No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21560.18989471236588026No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT21010.1850504594994037No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC20490.18047043860746226No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTACAAAACTCCGCTCTCTTT20270.17853273746087164No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC20180.17774004153726639No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT20060.17668311363912606No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19680.173336175295015No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19420.17104616484904428No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA19000.1673469172055531No Hit
ACCTTGGTCCCTCCGCCGAAAGAGAGCGGAGTTTTGTAATATTGTTGACAGTAATAAACTGCCACATCTTCAGC18330.1614457364409362No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT18140.1597722672688807No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA17800.15677763822414975No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17190.15140492140860304No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT17140.1509645347843779No Hit
GCAGAGGCCAGGCCAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAATACGGGGTCCCTGACCGATT17050.15017183886077265No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16880.1486745243384072No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTCTTATACAGCTTCAA16720.1472652871408867No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC16640.14656066854212652No Hit
ACCCGGGAATACGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGAGACAGACTTCACTCTCACCATCAGCAGC16530.1455918179688312No Hit
GCTGATGGTGAGAGTGAAGTCTGTCTCAGACCCGCTGCCACTGAATCGGT16490.1452395086694511No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16300.14356603949739555No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA16170.1424210342744102No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTCTTATACAGCTTCAACAACAAGAACTA16160.14233295694956516No Hit
GGAATACGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGAGACAGACT16160.14233295694956516No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG16140.14215680229987512No Hit
ATATTACAAAACTCCGCTCTCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT16040.1412760290514248No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16000.1409237197520447No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15850.1396025598793693No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGGTGA15740.138633709306074No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15430.13590331223587812No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT15290.13467022968804773No Hit
CTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA15240.13422984306382257No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC15110.13308483784083722No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC15080.13282060586630215No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC15040.13246829656692202No Hit
CCTTGGTCCCTCCGCCGAAAGAGAGCGGAGTTTTGTAATATTGTTGACAGTAATAAACTGCCACATCTTCAGC15010.13220406459238696No Hit
CTACGGGGACATCGTGGTGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG14890.1311471366942466No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14850.1307948273948665No Hit
CAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTA14610.12868097159858582No Hit
GGCCTCTGCTGGTACCAAGCTAAGTAGTTCTTGTTGTTGAAGCTGTATAA14290.12586249720354495No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA14220.12524595592962975No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT14200.1250698012799397No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG14190.12498172395509466No Hit
CCACTGAATCGGTCAGGGACCCCGTATTCCCGGGTAGATGCCCAGTAAATGAGCAACTTAGGAGGCTGGCCTGGC14110.12427710535633442No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14000.12330825478303913No Hit
GTAAATGAGCAACTTAGGAGGCTGGCCTGGCCTCTGCTGGTACCAAGCTA13820.12172286293582862No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGGTGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG13680.12048978038799822No Hit
GACCGATTCAGTGGCAGCGGGTCTGAGACAGACTTCACTCTCACCATCAG13440.11837592459171757No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT13380.11784746064264741No Hit
CTCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCAT13370.11775938331780238No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG13340.11749515134326727No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13290.11705476471904214No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC13160.11590975949605678No Hit
GGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC13130.1156455275215217No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13020.11467667694822639No Hit
CAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGA13010.11458859962338137No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12920.1137959036997761No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGC12740.1122105118525656No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC12620.11115358395442528No Hit
GCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGAGAGCGG12530.11036088803082No Hit
GTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGC12520.11027281070597499No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC12480.10992050140659487No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC12140.10692587236186392No Hit
CAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGG12090.10648548573763879No Hit
ACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAA12030.10595702178856861No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG11960.10534048051465343No Hit
ACTTAGCTTGGTACCAGCAGAGGCCAGGCCAGCCTCCTAAGTTGCTCATT11700.10305047006868269No Hit
TTCCCGGGTAGATGCCCAGTAAATGAGCAACTTAGGAGGCTGGCCTGGCCTCTGCTGGTACCAAGCTAAGTAGTT11610.10225777414507745No Hit
GAGACAGACTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGC11560.10181738752085231No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT11460.10093661427240204No Hit
GGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGA11450.10084853694755701No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT11430.10067238229786696No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG11370.10014391834879677No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTGTG451.9469221E-458.3728270
GTGGTAT2000.049.613241
TGGTATC2100.045.609312
GTATCAA17500.044.383071
GGTATCA6100.043.1907231
TATCAAC19900.038.676242
ATCAACG21050.036.8882943
TCAACGC21400.036.2849774
CAACGCA22150.035.0563665
TAGGCAA750.00241047135.02369770
AACGCAG22200.034.9774136
TTAGACG400.002565274534.5847139
AGAGTAC24150.031.3253611
CGCAGAG24650.031.2385488
ACGCAGA24800.031.179367
CAGAGTA24800.030.7803910
GTAAGAC450.004819602730.4062083
TCTCGCC14750.030.08214442
GCAGAGT25600.029.9508979
TCGCCCA15100.029.89337744
Produced by FastQC (version 0.11.5)