FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences916377
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24300.2651747042974671No Hit
GTATAATAATCTGCCTTTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT23270.2539347888478214No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG19550.21334014275783877No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18540.20231847809362305No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18130.19784433699230777No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17460.19053293568040228No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16030.1749280045221563No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15860.17307287284600115No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGTCAGGCGAGTCAGGACATTAGCGA15780.1721998697042811No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15520.16936260949369092No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15240.16630709849767072No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14960.16325158750165053No Hit
TTATTATACTGTTGACAGTAATATGCTGCAATATCTTCAGGCTGCAGGCTGTTGATAGTAAAAGTAAAATGTGTC14480.15801356865133018No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG13950.1522299228374348No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGTCAGGCGAGTCAGG13630.1487379102705546No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG13280.14491852152552934No Hit
GTAATATGCTGCAATATCTTCAGGCTGCAGGCTGTTGATAGTAAAAGTAA12940.1412082581732191No Hit
CAGTAATATGCTGCAATATCTTCAGGCTGCAGGCTGTTGATAGTAAAAGT12820.139898753460639No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT12130.13236910136330354No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12040.13138697282886846No Hit
AGTCAGGACATTAGCGACGATTTAAATTGGTATCAGCAGAAACCAGGGAA11990.13084134586529342No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11560.12614895397854814No Hit
GTGGGAGACAGAGTCACCATCACTTGTCAGGCGAGTCAGGACATTAGCGACGATTTAAATTGGTATCAGCAGAAA11110.12123831130637282No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11050.12058355895008277No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10850.11840105109578263No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC10840.11829192570306762No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10750.11730979716863257No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9840.10737938643156691No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9540.10410562465011672No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT9410.10268699454482162No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9280.10126836443952654No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG605.4078555E-946.19071240
CGTATGC803.274181E-1143.32993741
GTATCAA16250.039.672451
GTGGTAT2450.036.5854231
CTCGTAT709.455798E-734.6278439
CAACGCA19200.033.023865
AACGCAG19800.032.0231326
TAGACCG450.004648724630.6308235
AACTAGC450.004648724630.6308232
ACGCAGA21450.029.5598167
CAGAGTA21100.029.39688110
AGAGTAC21050.029.30300311
TATTAGA953.2355456E-729.0186752
CGCAGAG22100.028.6904098
CGAGACT1452.3646862E-1128.5292523
TCAACGC22250.028.4969924
ATCAACG22550.028.1178743
GTATTAG1254.1818566E-927.579781
CGGACTA500.007765953427.5767722
TGACTCG6700.025.8499770
Produced by FastQC (version 0.11.5)