FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005754974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences916377
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23450.2558990459166915No Hit
GTATAATAATCTGCCTTTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT22530.24585950978691082No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG18390.20068159720289794No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18040.1968622084578727No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16520.18027514876519163No Hit
TTATTATACTGTTGACAGTAATATGCTGCAATATCTTCAGGCTGCAGGCTGTTGATAGTAAAAGTAAAATGTGTC16520.18027514876519163No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15400.16805310478111082No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15200.1658705969268107No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG15140.16521584457052063No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14500.1582318194367602No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14340.15648581315332008No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14320.15626756236789008No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGTCAGGCGAGTCAGGACATTAGCGA13740.14993828959041966No Hit
GTAATATGCTGCAATATCTTCAGGCTGCAGGCTGTTGATAGTAAAAGTAA12970.14153563435136413No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12680.13837099796262892No Hit
GTGGGAGACAGAGTCACCATCACTTGTCAGGCGAGTCAGGACATTAGCGACGATTTAAATTGGTATCAGCAGAAA12530.1367341170719038No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGTCAGGCGAGTCAGG12520.1366249916791888No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG12390.1352063615738937No Hit
CAGTAATATGCTGCAATATCTTCAGGCTGCAGGCTGTTGATAGTAAAAGT12200.1331329791123086No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11980.1307322204725784No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11970.13062309507986342No Hit
AGTCAGGACATTAGCGACGATTTAAATTGGTATCAGCAGAAACCAGGGAA11490.1253850762295431No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10190.11119877517659217No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9990.10901626732229203No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9920.10825238957328698No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT9890.10792501339514195No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9560.10432387543554672No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9320.10170486601038654No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)