FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences775161
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCCAGGACGATCAACTTGGTCCCTCCGCCGAACACCCA35240.45461523477058313No Hit
GTAATAATCAGCCTCATCTTCGGACCGGAGACCGCTGATGGCCAGGGAGG32000.4128174663069995No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC26560.34263849703480953No Hit
GATTATTACTGTGCAACATTGGATGACAGCCTGCCTAGTTGGGTGTTCGG24870.32083657459547116No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGATCAACTTGGTCCCTC15990.20627972769527878No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT15420.19892641657668536No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG15080.1945402309971735No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG14790.1907990727087663No Hit
CCCCAGGGACGGCCCCCAAACTCCTCATCTATGGGAGTGAACAGCGGCCC13980.1803496305928704No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13290.17144825397562571No Hit
GACCCAGGACGATCAACTTGGTCCCTCCGCCGAACACCCAACTAGGCAGG12590.16241787190016008No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT12460.1607408009432879No Hit
CCCTCAGCCTCTGGGACCCCCGGACAGAGGGTCACCATCTCTTGTTCTGG12200.15738665902954352No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG12180.15712864811310165No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC11090.1430670531670195No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11070.1428090422505776No Hit
ACATTGGATGACAGCCTGCCTAGTTGGGTGTTCGGCGGAGGGACCAAGTTGATCGTCCTGGGTCAGCCCAAGG11050.14255103133413574No Hit
ATCCAATGTTGCACAGTAATAATCAGCCTCATCTTCGGACCGGAGACCGC10940.14113197129370542No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10930.1410029658354845No Hit
CCCCCGGACAGAGGGTCACCATCTCTTGTTCTGGAAGCGACTCCAACATC10460.13493970929910046No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC10430.13455269292443764No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG10350.13352064925867013No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGCTTGGAGGGTGTGGTGGTCTCCA9950.12836043092983263No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA9920.12797341455516983No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9400.1212651307276811No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGCGGTCTCCGGT9390.12113612526946016No Hit
GGAGTGAACAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG9240.11920104339614608No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9020.11636292331528547No Hit
CCCCTGAGGGCCGCTGTTCACTCCCATAGATGAGGAGTTTGGGGGCCGTCCCTGGGGATTGCTGGTACCAGTAGA8900.11481485781663421No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGATCAACTTGGTCCCTCCGCCGAACACCCAA8830.11391181960908765No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8610.11107369952822704No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC8590.11081568861178517No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC8500.10965463948779672No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC8490.1095256340295758No Hit
ATGTTGCACAGTAATAATCAGCCTCATCTTCGGACCGGAGACCGCTGATG8290.10694552486515704No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGATCAACTTGGTC8280.10681651940693611No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT8020.10346237749319173No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT7980.10294635566030798No Hit
GTGGTAGAGAAGACAGGATTCAGGGCAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA7890.10178530653631955No Hit
CAACTAGGCAGGCTGTCATCCAATGTTGCACAGTAATAATCAGCCTCATC7840.10114027924521486No Hit
GTTCACTCCCATAGATGAGGAGTTTGGGGGCCGTCCCTGGGGATTGCTGG7820.10088226832877299No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGCGGTCTCCGGTCCGAAGATG7820.10088226832877299No Hit
CTCTTGTTCTGGAAGCGACTCCAACATCGGAGCTAATTATGTCTACTGGTACCAGCAATCCCCAGGGACGGCCCC7790.10049525195411017No Hit
CCACCACACCCTCCAAGCAAAGCAACAACAAGTACGCGGCCAGCAGCTAC7780.10036624649588924No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG7760.10010823557944737No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTAA200.006992044452.42218862
CCCGTAT306.2862784E-446.0354358
GGTATCA5950.043.5349351
GTGGTAT1950.038.965631
GTATCAA12500.037.8533331
CTCGTAT501.8155483E-434.64501639
TATCAAC13700.033.770522
TCAACGC14350.032.2408454
ATCAACG14600.031.6887743
CAACGCA14850.031.1552965
AACGCAG14850.031.1552966
CGTGACG450.00434588431.05486561
GCTTAAT450.00447366330.87150247
CCCTATA450.004604395530.690292
TGGTATC2400.030.2107542
TCGTATG703.7242913E-529.7149640
AATGTTG16300.029.52614670
AGGGGGG1503.982859E-628.79430270
AGAGTAC16400.028.42126711
CAGAGTA16500.028.03976410
Produced by FastQC (version 0.11.5)